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caspase-6; MCH-2 protein (CASP6, MCH2)

Function: - role in the activation cascade of caspases responsible for apoptosis execution - cleaves poly(ADP-ribose) polymerase & lamins in vitro - cleaves DNA topoisomerase 1 - may participate in processing of amyloid precursor protein (APP) [2] - overexpression promotes programmed cell death - activation is suppressed by phosphorylation at Ser-257 - cleavages by caspase-3, caspase-8 or caspase-10 generate the two active subunits - strict requirement for Asp at position P1 & has a preferred cleavage sequence of Val-Glu-His-Asp-|- Structure: - heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kD (p18) & a 11 kD (p11) subunit - belongs to the peptidase C14 family Compartment: cytoplasm Alternative splicing: named isoforms=2

Interactions

molecular events

General

caspase; cysteinyl aspartate-specific proteinase; ICE/CED3 family protease phosphoprotein

Properties

SIZE: entity length = 293 aa MW = 33 kD COMPARTMENT: cytoplasm MOTIF: histidine residue {H121} cysteine residue {C163} Ser phosphorylation site {S257} STATE: active state

Database Correlations

OMIM 601532 UniProt P55212 Pfam PF00656 Entrez Gene 839 KEGG correlations ENZYME 3.4.22.59

References

  1. Martin SJ, Green DR. Protease activation during apoptosis: death by a thousand cuts? Cell. 1995 Aug 11;82(3):349-52. Review. No abstract available. PMID: 7634323
  2. Rampon C, Jiang CH, Dong H, Tang YP, Lockhart DJ, Schultz PG, Tsien JZ, Hu Y. Effects of environmental enrichment on gene expression in the brain. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23):12880-4. PMID: 11070096
  3. UniProt :accession P55212