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enzymatic reaction

The CPP32/YAMA ICE family cysteine protease cleaves & inactivates poly ADP-ribose polymerase.

Properties

GENERAL: catalytic reaction SPECIFIC: biochemical ligation sialylation active transport protein phosphorylation proteolytic cleavage template-directed reaction ENZYME: caspase-3 COMPARTMENT: cytoplasm MOTIF: Ser phosphorylation site {S26} histidine residue {H121} cysteine residue {C163} STATE: active state SUBSTRATE: poly ADP-ribose polymerase MOTIF: DNA-binding motif SITE: 1-371 MOTIF: nuclear translocation signal {206-208} <1/2SITE> nuclear translocation signal {220-225} <1/2SITE> BRCA1 C-terminal (BRCT) motif SITE: 384-460 automodification {372-523} MOTIF: binding site (15) SITE: 406-519 FOR-BINDING-OF: ADP ribose cofactor-binding site [524-1013] FOR-BINDING-OF: NAD active site SITE: 892 PRODUCT: peptide (SEVERAL)

References

  1. Martin SJ, Green DR. Protease activation during apoptosis: death by a thousand cuts? Cell. 1995 Aug 11;82(3):349-52. Review. No abstract available. PMID: 7634323