zonadhesin (ZAN)

Function: - binds in a species-specific manner to the zona pellucida of the egg - may be involved in gamete recognition &/or signaling - probably forms covalent oligomers Structure: - the MAM domains probably mediate sperm adhesion to the zona pellucida - during sperm migration through the reproductive tracts, the mucin-like domain might inhibit inappropriate trapping of spermatozoa or promoting adhesion to the oviductal isthmus - the VWFD domain 2 may mediate covalent oligomerization - contains 1 EGF-like domain - contains 3 MAM domains - contains 4 TIL (trypsin inhibitory-like) domains - contains 5 VWFC domains - contains 4 VWFD domains Compartment: - cell membrane; single-pass type 1 membrane protein. - exclusively on the apical region of the sperm head Alternative splicing: - named isoforms=7 - some isoforms may be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay Expression: - in testis, primarily in haploid spermatids

General

glycoprotein membrane protein

Properties

SIZE: entity length = 2812 aa MW = 306 kD COMPARTMENT: cellular membrane MOTIF: signal sequence {1-17} MAM domain {39-204} MOTIF: cysteine residue {*1} (2) MODIFICATION: cysteine residue {*2} cysteine residue {*2} (2) MODIFICATION: cysteine residue {*1} MAM domain {209-368} MOTIF: cysteine residue {*1} (2) MODIFICATION: cysteine residue {*2} cysteine residue {*2} (2) MODIFICATION: cysteine residue {*1} N-glycosylation site {N333} MAM domain {371-536} MOTIF: cysteine residue {*1} (2) MODIFICATION: cysteine residue {*2} cysteine residue {*2} (2) MODIFICATION: cysteine residue {*1} N-glycosylation site {N493} 66 X heptapeptide repeats (approximate) (mucin-like domain) {573-1041} TIL 1 {1044-1093} VWFC domain {1103-1148} MOTIF: N-glycosylation site {N1112} VWFD domain {1155-1371} MOTIF: N-glycosylation site {N1188} TIL 2 {1426-1479} VWFC domain {1480-1535} VWFD domain {1541-1758} MOTIF: N-glycosylation site {N1685} N-glycosylation site {N1804} TIL 3 {1812-1867} VWFC domain {1868-1924} MOTIF: N-glycosylation site {N1900} VWFD domain {1930-2144} MOTIF: N-glycosylation site {N1946} N-glycosylation site {N2203} TIL 4 {2211-2267} VWFC domain {2268-2329} VWFD domain {2330-2537} N-glycosylation site {N2542} VWFC domain {2652-2797} MOTIF: N-glycosylation site {N2701} EGF domain {2708-2744} cysteine residue {C2712} MODIFICATION: cysteine residue {C2723} cysteine residue {C2717} MODIFICATION: cysteine residue {C2732} cysteine residue {C2723} MODIFICATION: cysteine residue {C2712} cysteine residue {C2732} MODIFICATION: cysteine residue {C2717} cysteine residue {C2734} MODIFICATION: cysteine residue {C2743} cysteine residue {C2743} MODIFICATION: cysteine residue {C2734} transmembrane domain {2758-2778}

Database Correlations

OMIM 602372 UniProt Q9Y493 PFAM correlations Entrez Gene 7455 Kegg hsa:7455

References

UniProt :accession Q9Y493