nuclease-sensitive element-binding protein 1; Y-box-binding protein 1; Y-box transcription factor; YB-1; CCAAT-binding transcription factor I subunit A; CBF-A; enhancer factor I subunit A; EFI-A; DNA-binding protein B; DBPB (YBX1, NSEP1, YB1)

Function: - binds to splice sites in pre-mRNA & regulates splice site selection - binds & stabilizes cytoplasmic mRNA - role in regulation of translation by modulating the interaction between mRNA & eukaryotic initiation factors - binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes - regulates transcription of numerous genes - promotes separation of DNA strands that contain mismatches or are modified by cisplatin - has endonucleolytic activity & can introduce nicks or breaks into double-stranded DNA (in vitro) - may play a role in DNA repair component of cytoplasmic messenger ribonucleoprotein particles (mRNPs) - interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN & NCL - can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B - homodimer in the presence of ATP - unphosphorylated protein is retained in the cytoplasm - cleaved by a 20S proteasomal protease in response to agents that damage DNA a) cleavage takes place in the absence of ubiquitination & ATP b) resulting N-terminal fragment accumulates in the nucleus (putative) Structure: contains 1 CSD (cold-shock) domain Compartment: - cytoplasm, nucleus - shuttles between nucleus & cytoplasm - predominantly cytoplasmic in proliferating cells - cytotoxic stress & DNA damage enhance translocation to the nucleus

General

DNA-binding protein endodeoxyribonuclease phosphoprotein splicing factor; snRNP-associated protein transcriptional silencer (transcriptional repressor)

Properties

SIZE: entity length = 324 aa MW = 36 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: ss-DNA interaction {15-71} cold shock NAME: cold shock SITE: 61-125 MOTIF: Ser phosphorylation site {S102} Tyr phosphorylation site {Y162} Ser phosphorylation site {S165} Ser phosphorylation site {S167} Ser phosphorylation site {S174} Ser phosphorylation site {S176} peptide motif {219-220} Ser phosphorylation site {S314}

Database Correlations

OMIM 154030 UniProt P67809 Pfam PF00313 Kegg hsa:4904

References

UniProt :accession P67809