WAS/WASL-interacting protein family member 2 (WIP-related protein, WASP-interacting protein-related protein, WIP- & CR16-homologous protein, WIPF2, WICH, WIRE, PP10631)

Function: - role in formation of cell surface protrusions downstream of activated PDGFB receptors - role in actin-microspike formation through cooperation with WASL - may cooperate with WASP & WASL to induce mobilization & reorganization of the actin filament system - interacts with WASL & WASP, interaction results in cytoplasmic relocation of these 2 proteins along actin filaments - interacts with NCK2 resulting in the localization to sites of focal adhesions - no interaction observed with WASF2 & WASF3 Structure: - contains 1 WH2 domain - access to profilin-binding site is masked in full-length protein Compartment: - cytoplasm, cytoskeleton - localized to stress fibers & bundles of actin filaments Alternative splicing: named isoforms=2 Expression: - expressed mainly in brain, colon, lung, stomach (at protein level) - ubiquitously expressed, with high expression in brain, kidney, lung, & placenta

Properties

SIZE: MW = 46 kD entity length = 440 aa COMPARTMENT: cytoplasm MOTIF: proline-rich region SITE: 2-17 MOTIF: proline residue (SEVERAL) WH2 {36-53} MOTIF: Binds actin {49-52} proline-rich region SITE: 177-182 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 250-254 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 296-299 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 311-314 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 325-330 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 340-345 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 359-363 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 370-377 MOTIF: proline residue (SEVERAL)

Database Correlations

UniProt Q8TF74 Pfam PF02205

References

UniProt :accession Q8TF74