WAP, kazal, immunoglobulin, kunitz & NTR domain-containing protein 2 (WAP, follistatin, immunoglobulin, kunitz & NTR domain-containing-related protein, WFIKKN-related protein, growth & differentiation factor-associated serum protein 1, GASP-1, hGASP-1, WFIKKN2, GASP1, WFIKKNRP, UNQ9235/PRO31996)

Function: - protease-inhibitor - multiple distinct protease inhibitor domains - probably has serine protease- & metalloprotease-inhibitor activity - inhibits the biological activity of mature myostatin, but not activin (putative) - interacts with both mature & propeptide myostatin/MSTN Structure: - belongs to the WFIKKN family - contains 2 BPTI/Kunitz inhibitor domains - contains 1 Ig-like C2-type domain (immunoglobulin-like) - contains 1 Kazal-like domain - contains 1 NTR domain - contains 1 WAP domain Compartment: secreted (putative) Expression: - primarily expressed in ovary, testis & brain, but not in liver. - in fetal tissues, primarily expressed in brain, skeletal muscle, thymus & kidney

General

glycoprotein secreted protein serine protease inhibitor; serpin

Properties

SIZE: entity length = 576 aa MW = 64 kD COMPARTMENT: extracellular compartment INHIBITS: serine protease MOTIF: signal sequence {1-34} WAP {42-91} MOTIF: cysteine residue {C46} MODIFICATION: cysteine residue {C79} cysteine residue {C62} MODIFICATION: cysteine residue {C83} cysteine residue {C66} MODIFICATION: cysteine residue {C78} cysteine residue {C72} MODIFICATION: cysteine residue {C88} cysteine residue {C78} MODIFICATION: cysteine residue {C66} cysteine residue {C79} MODIFICATION: cysteine residue {C46} cysteine residue {C83} MODIFICATION: cysteine residue {C62} cysteine residue {C88} MODIFICATION: cysteine residue {C72} Kazal-type serine protease inhibitor domain {133-175} MOTIF: cysteine residue {X+0} MODIFICATION: cysteine residue {X+X4} cysteine residue {X+X1} MODIFICATION: cysteine residue {X+X3} cysteine residue {X+X2} MODIFICATION: cysteine residue {X+X5} cysteine residue {X+X3} MODIFICATION: cysteine residue {X+X1} cysteine residue {X+X4} MODIFICATION: cysteine residue {X+0} cysteine residue {X+X5} MODIFICATION: cysteine residue {X+X2} cysteine residue {C134} MODIFICATION: cysteine residue {C164} cysteine residue {C138} MODIFICATION: cysteine residue {C157} Reactive bond {140-141} cysteine residue {C146} MODIFICATION: cysteine residue {C175} cysteine residue {C157} MODIFICATION: cysteine residue {C138} cysteine residue {C164} MODIFICATION: cysteine residue {C134} cysteine residue {C175} MODIFICATION: cysteine residue {C146} FOR-BINDING-OF: serine protease immunoglobulin superfamily domain {210-303} MOTIF: cysteine residue {C231} MODIFICATION: cysteine residue {C287} cysteine residue {C287} MODIFICATION: cysteine residue {C231} N-glycosylation site {N319} BPTI/Kunitz inhibitor 1 {328-378} MOTIF: cysteine residue {C328} MODIFICATION: cysteine residue {C378} cysteine residue {C337} MODIFICATION: cysteine residue {C361} cysteine residue {C353} MODIFICATION: cysteine residue {C374} cysteine residue {C361} MODIFICATION: cysteine residue {C337} cysteine residue {C374} MODIFICATION: cysteine residue {C353} cysteine residue {C378} MODIFICATION: cysteine residue {C328} BPTI/Kunitz inhibitor 2 {386-436} MOTIF: cysteine residue {C386} MODIFICATION: cysteine residue {C436} cysteine residue {C395} MODIFICATION: cysteine residue {C419} cysteine residue {C411} MODIFICATION: cysteine residue {C432} cysteine residue {C419} MODIFICATION: cysteine residue {C395} cysteine residue {C432} MODIFICATION: cysteine residue {C411} cysteine residue {C436} MODIFICATION: cysteine residue {C386} NTR {445-566} MOTIF: cysteine residue {C445} MODIFICATION: cysteine residue {C515} cysteine residue {C448} MODIFICATION: cysteine residue {C517} cysteine residue {C459} MODIFICATION: cysteine residue {C566} cysteine residue {C515} MODIFICATION: cysteine residue {C445} cysteine residue {C517} MODIFICATION: cysteine residue {C448} N-glycosylation site {N519} cysteine residue {C566} MODIFICATION: cysteine residue {C459}

Database Correlations

UniProt Q8TEU8 PFAM correlations

References

UniProt :accession Q8TEU8