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upstream stimulatory factor 2; class B basic helix-loop-helix protein 12; bHLHb12; FOS-interacting protein; FIP; major late transcription factor 2; upstream transcription factor 2 (USF2, BHLHB12)
Function:
- transcription factor & DNA-binding protein
- binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in a variety of viral & cellular promoters
- efficient DNA binding requires dimerization with another bHLH protein
- binds DNA as an homodimer or a heterodimer (USF1/USF2)
- in vivo, the USF1/USF2A heterodimer represents over 66% of the USF binding activity whereas the USF1 & USF2A homodimers represent less than 10%
- the USF1/USF2B heterodimer accounts for almost 15% in some cells
Structure: contains 1 basic helix-loop-helix (bHLH) domain
Compartment: nucleus
Alternative splicing:
- named isoforms=3; USF2A, USF2A-delta-H, USF2B
- additional isoforms seem to exist
Expression: ubiquitous
General
basic helix loop helix (bHLH) family transcription factor
phosphoprotein
Properties
SIZE: entity length = 346 aa
MW = 37 kD
COMPARTMENT: cell nucleus
MOTIF: alanine-rich region {11-20}
MOTIF: alanine residue (SEVERAL)
DNA-binding motif
SITE: 235-248
MOTIF: arginine-rich region {245-248}
MOTIF: arginine residue (SEVERAL)
helix-loop-helix
NAME: helix-loop-helix
SITE: 249-291
FOR-BINDING-VIA: helix-loop-helix
Thr phosphorylation site {T293}
leucine zipper
SITE: 307-328
FOR-BINDING-VIA: leucine zipper
Database Correlations
OMIM 600390
UniProt Q15853
Pfam PF00010
Entrez Gene 7392
Kegg hsa:7392
References
- UniProt :accession Q15853
- OMIM :accession 600390