UMP-CMP kinase 2, mitochondrial (CMPK2)

Function: - may participate in dUTP & dCTP synthesis in mitochondria - is able to phosphorylate dUMP, dCMP, CMP, UMP & monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU & FdUrd with ATP as phosphate donor - efficacy is highest for dUMP followed by dCMP; CMP & UMP are poor substrates - may be involved in mtDNA depletion caused by long term treatment with ddC or other pyrimidine analogs ATP + dCMP ADP + dCDP Kinetic parameters: - KM=3.09 mM for CMP - KM=6.3 mM for UMP - KM=1.31 mM for dCMP - KM=0.1 mM for dUMP - Vmax=1.64 umol/min/mg enzyme towards CMP - Vmax=0.19 umol/min/mg enzyme towards UMP - Vmax=1.77 umol/min/mg enzyme towards dCMP - Vmax=0.48 umol/min/mg enzyme towards dUMP Structure: belongs to the thymidylate kinase family Compartment: mitochondria Alternative splicing: named isoforms=4 Expression: - among all investigated tumors, leukemia cells show the most abundant expression

General

enzyme mitochondrial protein

Properties

SIZE: entity length = 449 aa MW = 49 kD COMPARTMENT: mitochondria MOTIF: ATP-binding site NAME: ATP-binding site SITE: 259-266 coiled coil {380-412}

Database Correlations

UniProt Q5EBM0 Pfam PF02223 ENZYME 2.7.4.14

References

UniProt :accession Q5EBM0