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E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; hyperplastic discs protein homolog; hHYD; Progestin-induced protein (UBR5, EDD, EDD1, HYD, KIAA0896)
Function:
- E3 ubiquitin-protein ligase, component of the N-end rule pathway
- recognizes & binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination & subsequent degradation (putative)
- may be involved in maturation &/or transcriptional regulation of mRNA
- may play a role in control of cell cycle progression
- may have tumor suppressor function
- regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response
- role in extraembryonic development
- protein modification; protein ubiquitination
- phosphorylated upon DNA damage, probably by ATM or ATR
- binds TOPBP1
Structure:
- contains 1 HECT domain
- contains 1 PABC domain
- contains 1 UBR-type Zn+2 finger
- a Cys is required for ubiquitin-thioester formation
Compartment: nucleus
Expression:
- widely expressed
- most abundant in testis
- expressed at high levels in brain, pituitary & kidney
General
nuclear protein
phosphoprotein
E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein
zinc finger protein
Properties
SIZE: entity length = 2799 aa
MW = 309 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S139}
Ser phosphorylation site {S287}
Ser phosphorylation site {S327}
Ser phosphorylation site {S598}
glutamate-rich region {940-945}
MOTIF: glutamate residue (SEVERAL)
serine-rich region {980-985}
MOTIF: serine residue (SEVERAL)
Zinc finger
NAME: Zinc finger
SITE: 1177-1245
EFFECTOR-BOUND: Zn+2
Ser phosphorylation site {S1308}
serine-rich region {1528-1537}
MOTIF: serine residue (SEVERAL)
Ser phosphorylation site {S1549}
Ser phosphorylation site {S1551}
serine-rich region {1671-1681}
MOTIF: serine residue (SEVERAL)
Tyr phosphorylation site {Y1746}
alanine-rich region {1762-1768}
MOTIF: alanine residue (SEVERAL)
Thr phosphorylation site {T1969}
acidic region {1986-1997}
MOTIF: acidic residue (SEVERAL)
proline-rich region
SITE: 2036-2059
MOTIF: proline residue (SEVERAL)
Ser phosphorylation site {S2071}
glutamate-rich region {2329-2348}
MOTIF: glutamate residue (SEVERAL)
PABC {2377-2454}
HECT domain {2462-2799}
MOTIF: Ser phosphorylation site {S2486}
acidic region {2489-2500}
MOTIF: acidic residue (SEVERAL)
proline-rich region
SITE: 2737-2757
MOTIF: proline residue (SEVERAL)
cysteine residue {C2768}
Database Correlations
OMIM 608413
UniProt O95071
PFAM correlations
Entrez Gene 51366
Kegg hsa:51366
References
UniProt :accession O95071