ubiquitin-like PHD & RING finger domain-containing protein 2; E3 ubiquitin-protein ligase UHRF2; EC=6.3.2.-; Np95/ICBP90-like RING finger protein; Np95-like RING finger protein; nuclear protein 97; nuclear zinc finger protein Np97; RING finger protein 107; ubiquitin-like-containing PHD & RING finger domains protein 2 (UHRF2, NIRF, RNF107)

Function: 1) E3 ubiquitin ligase protein - ubiquitin conjugation, 3rd step - ubiquitinates cyclins, CCND1 & CCNE1 (phosphorylation independent) & induces G1 arrest 2) G1/S transition - overexpression causes G1 phase cell arrest 3) binds methylated CpG containing oligonucleotides 4) interacts with PCNP, HDAC1, inactive CDK2 - ubiquitinates PCNP leading to its degradation by the proteasome 5) component of UHRF2/CDK2/CCNE1 complex 6) may be autoubiquitinated, leading to proteasomal degradation 7) phosphorylation may be mediated by CDK2 8) component of a complex at least composed of UHRF2, CDK2 & CCNE1 - interaction with CCNE1 ubiquitinates CCNE1 9) interaction with CCND1 biquitinates CCND1 10) interacts with p53/TP53 & RB1 Structure: - contains 1 PHD-type Zn⁺² finger - contains 2 RING-type Zn⁺² fingers - contains 1 ubiquitin-like domain - contains 1 YDG domain Compartment: nucleus Expression: - up-regulated in proliferating non tumor cells - down-regulated by growth arrest & differentiation Pathology: - chromosomal deletion involving UHRF2 is found in multiple kinds of malignancies including - brain cancer - breast cancer - stomach cancer - kidney cancer - hematopoietic malignancy - lung cancer

General

nuclear protein phosphoprotein ring finger protein E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: entity length = 802 aa MW = 90 kD COMPARTMENT: cell nucleus MOTIF: Ubiquitin-like {1-78} histone H3 interaction {117-311} MOTIF: PCNP interaction {194-288} Zn finger PHD-type NAME: Zn finger PHD-type SITE: 344-395 EFFECTOR-BOUND: Zn+2 domain {414-644} MOTIF: YDG {448-612} Ser phosphorylation site {S667} cysteine residue {C704} MODIFICATION: cysteine residue {CINTERCHAIN} RING-finger {733-772} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif

Database Correlations

UniProt Q96PU4 PFAM correlations Entrez Gene 115426 Kegg hsa:115426

References

UniProt :accession Q96PU4