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SUMO-activating enzyme subunit 2; anthracycline-associated resistance ARX; ubiquitin-like 1-activating enzyme E1B (UBA2 SAE2 UBLE1B HRIHFB2115)

Function: - heterodimer of SAE1 & UBA2/SAE2 acts as a E1 ligase for SUMO1, SUMO2, SUMO3, & probably SUMO4 - enzyme catalytic subunit - mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein & a conserved active site Cys on UBA2/SAE2 - protein modification; protein sumoylation - heterodimer of SAE1 & UBA2/SAE2 corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1 - interacts with UBE2I Structure: belongs to the ubiquitin-activating E1 family Compartment: nucleus Expression: - expression induced by c-Myc Laboratory: - SUMO-activating enzyme subunit 2 Ab in serum Notes: - SAE2 inhibition switches a transcriptional subprogram of c-Myc from activated to repressed [2] - AE2 inhibition produces defects in the mitotic spindles of cancerous cells triggered to divide by c-Myc activation [2]

Related

small ubiquitin-related modifier 1; SUMO-1; sentrin; ubiquitin-like protein SMT3C; SMT3 homolog 3; ubiquitin-homology domain protein PIC1; ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1 (SUMO1, SMT3C, SMT3H3, UBL1, OK/SW-cl43)

General

enzyme catalytic subunit nuclear protein phosphoprotein

Properties

SIZE: entity length = 640 aa MW = 71 kD COMPARTMENT: cell nucleus MOTIF: ATP-binding site NAME: ATP-binding site SITE: 24-29 ATP-binding site NAME: ATP-binding site SITE: 48-48 ATP-binding site NAME: ATP-binding site SITE: 56-59 ATP-binding site NAME: ATP-binding site SITE: 72-72 ATP-binding site NAME: ATP-binding site SITE: 95-96 ATP-binding site NAME: ATP-binding site SITE: 117-122 Zn+2-binding site SITE: 158-158 Zn+2-binding site SITE: 161-161 cysteine residue {C173} Zn+2-binding site SITE: 441-441 Zn+2-binding site SITE: 444-444 Ser phosphorylation site {S592}

Database Correlations

OMIM 613295 UniProt Q9UBT2 PFAM correlations Entrez Gene 10054 Kegg hsa:10054

References

  1. UniProt :accession Q9UBT2
  2. Kessler JD et al. A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 2012 Jan 20; 335:348. PMID: 22157079

Component-of

ubiquitin-like 1 [UBL1] activating enzyme E1 (SUMO-1 activating enzyme, UBLE1 ligase)