Contents

Search

ubiquitin-like 1 [UBL1] activating enzyme E1 (SUMO-1 activating enzyme, UBLE1 ligase)

Function: - acts as a E1 ligase for SUMO1, SUMO2, SUMO3, & probably SUMO4 - mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein & a conserved active site Cys on UBA2/SAE2 - protein modification; protein sumoylation Structure: - heterodimer of SAE1 & UBA2/SAE2 corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1 Pathology: - low levels of UBLE1 ligase in human breast cancer with activated c-Myc correlate with better survival [2]

Related

small ubiquitin-related modifier 1; SUMO-1; sentrin; ubiquitin-like protein SMT3C; SMT3 homolog 3; ubiquitin-homology domain protein PIC1; ubiquitin-like protein UBL1; GAP-modifying protein 1; GMP1 (SUMO1, SMT3C, SMT3H3, UBL1, OK/SW-cl43)

General

ligase or synthetase molecular complex

Properties

MOTIF: active site SUBUNITS: SUMO-activating enzyme subunit 1 MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S185} SUMO-activating enzyme subunit 2 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 24-29 ATP-binding site NAME: ATP-binding site SITE: 48-48 ATP-binding site NAME: ATP-binding site SITE: 56-59 ATP-binding site NAME: ATP-binding site SITE: 72-72 ATP-binding site NAME: ATP-binding site SITE: 95-96 ATP-binding site NAME: ATP-binding site SITE: 117-122 Zn+2-binding site SITE: 158-158 Zn+2-binding site SITE: 161-161 cysteine residue {C173} Zn+2-binding site SITE: 441-441 Zn+2-binding site SITE: 444-444 Ser phosphorylation site {S592}

References

  1. UniProt :accession Q9UBE0, Q9UBT2
  2. Kessler JD et al. A SUMOylation-dependent transcriptional subprogram is required for Myc-driven tumorigenesis. Science 2012 Jan 20; 335:348. PMID: 22157079

Components

SUMO-activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A (SAE1, AOS1, SUA1, UBLE1A) SUMO-activating enzyme subunit 2; anthracycline-associated resistance ARX; ubiquitin-like 1-activating enzyme E1B (UBA2 SAE2 UBLE1B HRIHFB2115)