ubiquitin ligase protein MIB2; mind bomb homolog 2; zinc finger ZZ type with ankyrin repeat domain protein 1; skeletrophin; novelzin; novel zinc finger protein; NF-kappa-B-activating protein 002N (MIB2, DIP1, KIAA1323, ZZANK2)

Function: 1) mediates ubiquitination of delta proteins 2) regulates delta1-mediated Notch signaling by ubiquitinating the intracellular domain of delta1, leading endocytosis 3) ubiquitin conjugation, 3rd step 4) ubiquitinated Compartment: - cytoplasm - localizes with endosomal compartment Alternative splicing: named isoforms=6 Expression: - expressed in skeletal muscle > heart, brain, kidney Pathology: 1) down-regulated in many primary skin melanomas 2) down-regulation may result from methylation of gene

General

ankyrin repeat domain protein (ANKRD) E3 ubiquitin ligase; ubiquitin-ligating enzyme E3; N end-recognizing protein

Properties

SIZE: MW = 110 kD entity length = 1013 aa COMPARTMENT: endosome MOTIF: C2 domain {70-138} MOTIF: binding site FOR-BINDING-OF: phospholipid Ca+2-binding site Zinc finger NAME: Zinc finger SITE: 143-190 EFFECTOR-BOUND: Zn+2 C2 domain {218-285} MOTIF: binding site FOR-BINDING-OF: phospholipid Ca+2-binding site ankyrin repeat NAME: ankyrin repeat (9) SITE: 522-856 RING-finger {890-925} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif RING-finger {969-1002} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif

Database Correlations

UniProt Q96AX9 PFAM correlations ENZYME 6.3.2.19

References

UniProt :accession Q86YT6