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ubiquitin-conjugating enzyme E2 L3; L-UBC; UbcH7; ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2-F1; ubiquitin-protein ligase L3 (UBE2L3, UBE2L1, UBCE7, UBCH7)

Function: - ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type & RBR family E3 ubiquitin-protein ligases - does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 & ARIH1, that function like function like RING-HECT hybrids - accepts ubiquitin from the E1 complex & catalyzes its covalent attachment to other proteins - in vitro catalyzes Lys-11-linked polyubiquitination - involved in the selective degradation of short-lived & abnormal proteins - down-regulated during the S-phase - role in progression through the cell cycle - regulates transcriptional activity of genes encoding nuclear hormone receptors - may play a role in myelopoiesis - protein modification; protein ubiquitination - ubiquitinated - alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation - interacts with PARK2 - role in ubiquitination & degradation of misfolded proteins - interacts with UBE3A - interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B & RNF144B - interacts with ARIH1 - interacts with ARIH2 (via RING-type 1) - interacts with NCOA1 - interaction regulates transcriptional activity of progesterone receptor gene - may interact with NR3C1 Structure: - in contrast to other ubiquitin-conjugating enzymes E2, residues essential for lysine reactivity are absent: - Pro & His are present instead of Asp in positions 88 & 119, respectively - belongs to the ubiquitin-conjugating enzyme family Compartment: - nucleus, cytoplasm Expression: - ubiquitous - highest expression in testis Pathology: - used by the papillomavirus HPV-16 E6 protein to ubiquitinate p53/TP53 Notes: - 2 genes for ubiquitin-conjugating proteins UBE2L1/UBE2L3 are identical except that the E2L1 gene is intronless, while the E2L3 coding sequence is interrupted by 3 introns [1] - UBE2L1, UBE2L2 & UBE2L4 are most likely pseudogenes & the only expressed member of this subfamily seems to be UBE2L3 [3]

General

nuclear protein ubiquitin-conjugating protein E2 (ubiquitin carrier protein)

Properties

SIZE: entity length = 154 aa MW = 18 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: cysteine residue {C86}

Database Correlations

OMIM 603721 UniProt P68036 Pfam PF00179 Entrez Gene 7332 Kegg hsa:7332 ENZYME 6.3.2.19

References

  1. UniProt :accession P68036
  2. OMIM :accession 600012
  3. Ardley HC et al Promoter analysis of the human ubiquitin-conjugating enzyme gene family UBE2L1-4, including UBE2L3 which encodes UbcH7 Biochim Biophys Acta. 2000 Apr 25;1491(1-3):57-64. PMID: 10760570