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ubiquitin carboxyl-terminal hydrolase 3; deubiquitinating enzyme 3; ubiquitin thiolesterase 3; ubiquitin-specific-processing protease 3 (USP3)
Function:
- deubiquitinates monoubiquitinated target proteins including histone H2A & H2B
- required for proper progression through S phase & subsequent mitotic entry
- may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites
- associates with the chromatin
- thiol-dependent hydrolysis of ester, thioester, amide, peptide & isopeptide bonds formed by the C-terminal Gly of ubiquitin
- interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A
Structure:
- both protease activity & an intact Zn+2 finger are required for H2A monodeubiquitination
- belongs to the peptidase C19 family, USP3 subfamily
- contains 1 UBP-type Zn+2 finger
Compartment:
- nucleus
- localizes preferentially with monoubiquitinated H2A to chromatin
Expression:
- expressed in all tissues examined
- strongest expression in pancreas
Related
ubiquitin (UBCEP2, UBB, UBC)
General
nuclear protein
phosphoprotein
ubiquitin C-terminal hydrolase; UCH; ubiquitin thiolesterase; ubiquitin-specific processing protease; deubiquitinating enzyme
zinc finger protein
Properties
SIZE: entity length = 520 aa
MW = 59 kD
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
Zinc finger
NAME: Zinc finger
SITE: 27-104
EFFECTOR-BOUND: Zn+2
Thr phosphorylation site {T141}
cysteine residue {C168}
histidine residue {H471}
Database Correlations
OMIM 604728
UniProt Q9Y6I4
PFAM correlations
Entrez Gene 9960
Kegg hsa:9960
ENZYME 3.4.19.12
References
- UniProt :accession Q9Y6I4
- OMIM :accession 604728