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ubiquitin carboxyl-terminal hydrolase 16; deubiquitinating enzyme 16; ubiquitin thiolesterase 16; ubiquitin-processing protease UBP-M; ubiquitin-specific-processing protease 16 (USP16)
Function:
- specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator
- deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at Ser-10 of histone H3, & is required for chromosome segregation when cells enter into mitosis
- regulates Hox gene expression via histone H2A deubiquitination
- prefers nucleosomal substrates
- does not deubiquitinate histone H2B
- thiol-dependent hydrolysis of ester, thioester, amide, peptide & isopeptide bonds formed by the C-terminal Gly of ubiquitin
- phosphorylated at the onset of mitosis & dephosphorylated during the metaphase/anaphase transition
- the phosphorylated form of the protein is also enzymatically active
Structure:
- homotetramer
- the UBP-type Zn+2 finger binds 3 Zn+2 that form a pair of cross-braced ring fingers encapsulated within a third Zn+2 finger in the primary structure
- recognizes the C-terminal tail of free ubiquitin
- belongs to the peptidase C19 family, USP16 subfamily
- contains 1 UBP-type Zn+2 finger
Compartment: nucleus (probable)
Alternative splicing: named isoforms=5
Expression:
- present in all the tissues examined including fetal brain, lung, liver, kidney, & adult heart, brain, placenta, lung, liver, skeletal muscle, kidney & pancreas
Pathology:
- chromosomal inversion inv(21)(q21;q22) involving USP16 with RUNX1/AML1 is a cause of chronic myelomonocytic leukemia
Related
ubiquitin (UBCEP2, UBB, UBC)
General
nuclear protein
phosphoprotein
ubiquitin C-terminal hydrolase; UCH; ubiquitin thiolesterase; ubiquitin-specific processing protease; deubiquitinating enzyme
zinc finger protein
Properties
SIZE: entity length = 823 aa
MW = 94 kD
COMPARTMENT: cell nucleus
MOTIF: Zn+2-binding site
SITE: 24-24
Zn+2-binding site
SITE: 26-26
Zn+2-binding site
SITE: 48-48
Zn+2-binding site
SITE: 51-51
Zinc finger
NAME: Zinc finger
SITE: 60-125
EFFECTOR-BOUND: Zn+2
MOTIF: Zn+2-binding site
SITE: 74-74
Zn+2-binding site
SITE: 77-77
Zn+2-binding site
SITE: 82-82
Zn+2-binding site
SITE: 88-88
Zn+2-binding site
SITE: 90-90
Zn+2-binding site
SITE: 94-94
Zn+2-binding site
SITE: 103-103
Zn+2-binding site
SITE: 116-116
Zn+2-binding site
SITE: 119-119
cysteine residue {C205}
Ser phosphorylation site {S415}
Ser phosphorylation site {S552}
Thr phosphorylation site {T554}
histidine residue {H758}
Database Correlations
OMIM 604735
UniProt Q9Y5T5
PFAM correlations
Entrez Gene 10600
Kegg hsa:10600
ENZYME 3.4.19.12
References
- UniProt :accession Q9Y5T5
- OMIM :accession 604735