non-receptor tyrosine-protein kinase TNK1; CD38 negative kinase 1 (TNK1)

Function: - role in negative regulation of cell growth - tumor suppressor properties - negative regulatory role in the Ras-MAPK pathway - may function in signaling pathways utilized broadly during fetal development & more selectively in adult tissues & in cells of the lymphohematopoietic system - could specifically be involved in phospholipid signaling - autophosphorylated on Tyr - interacts with SH3 domain of PLCG1 via its Pro-rich domain Structure: - belongs to the protein kinase superfamily, Tyr protein kinase family - contains 1 protein kinase domain - contains 1 SH3 domain Compartment: - cytoplasm, membrane Alternative splicing: named isoforms=2 Expression: - expressed in umbilical cord blood, bone marrow & adult blood cells - expressed in several leukemia cell lines - highly expressed in fetal blood, brain, lung, liver & kidney - expressed at lower levels in adult prostate, testis, ovary, small intestine & colon - not expressed in adult lung, liver, kidney or brain

Properties

SIZE: entity length = 666 aa MW = 72 kD COMPARTMENT: cytoplasm STATE: active state MOTIF: Ser phosphorylation site {S60} Ser phosphorylation site {S96} kinase domain SITE: 116-377 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 122-130 ATP-binding site NAME: ATP-binding site SITE: 148-148 aspartate residue {D245} Ser phosphorylation site {S255} Tyr phosphorylation site {Y277} src homology 3 [SH3] domain SITE: 381-444 MOTIF: Ser phosphorylation site {S411} Ser phosphorylation site {S468} Ser phosphorylation site {S502} Ser phosphorylation site {S508} proline-rich region SITE: 511-573 MOTIF: proline residue (SEVERAL) Thr phosphorylation site {T514} Ser phosphorylation site {S518} Ser phosphorylation site {S519} Ser phosphorylation site {S543} Ser phosphorylation site {S582} Tyr phosphorylation site {Y661}

Database Correlations

OMIM 608076 UniProt Q13470 Pfam PF07714 Entrez Gene 8711 Kegg hsa:8711 ENZYME 2.7.10.2

References

UniProt :accession Q13470