tyrosine protein kinase 7; colon carcinoma kinase 4; CCK-4; protein tyrosine kinase 7; pseudo tyrosine kinase receptor 7; tyrosine protein kinase-like 7 (PTK7, CCK4)

Function: - inactive tyrosine kinase involved in Wnt signaling pathway - component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) & the canonical Wnt signaling pathway - functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization & apoptosis - role in embryogenesis, epithelial tissue organization & angiogenesis - MMP14 cleaves PTK7 between Pro-621 & Leu-622 generating an N-terminal soluble (70 kDa) fragment & a membrane C-terminal (50 kDa) fragment - proteolysis by MMP14 regulates PTK7 function in the non-canonical Wnt signaling pathway - interacts with CTNNB1 Structure: - belongs to the protein kinase superfamily, Tyr protein kinase family, insulin receptor subfamily - contains 7 Ig-like C2-type domains - contains 1 protein kinase domain Compartment: - membrane - single-pass type 1 membrane protein - cell junction - colocalizes with MMP14 at cell junctions - also localizes at the leading edge of migrating cells Alternative splicing: - named isoforms=5 - at least 1 isoform may be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay Expression: - highly expressed in lung, liver, pancreas, kidney, placenta & melanocytes - weakly expressed in thyroid gland, ovary, brain, heart & skeletal muscle - also expressed in erythroleukemia cells - not expressed in colon - higher expression in cell lines established from normal non-tumorigenic tissues compared to cell lines established from highly metastatic invasive carcinomas (at protein level)

Properties

SIZE: entity length = 1070 aa MW = 118 kD COMPARTMENT: cellular membrane STATE: active state MOTIF: signal sequence {1-30} immunoglobulin superfamily domain {31-120} MOTIF: cysteine residue {C53} MODIFICATION: cysteine residue {C101} cysteine residue {C101} MODIFICATION: cysteine residue {C53} N-glycosylation site {N116} immunoglobulin superfamily domain {128-218} MOTIF: cysteine residue {C150} MODIFICATION: cysteine residue {C200} N-glycosylation site {N175} N-glycosylation site {N184} cysteine residue {C200} MODIFICATION: cysteine residue {C150} N-glycosylation site {N214} immunoglobulin superfamily domain {225-317} MOTIF: cysteine residue {C246} MODIFICATION: cysteine residue {C301} N-glycosylation site {N268} N-glycosylation site {N283} cysteine residue {C301} MODIFICATION: cysteine residue {C246} immunoglobulin superfamily domain {309-407} MOTIF: cysteine residue {C343} MODIFICATION: cysteine residue {C391} cysteine residue {C391} MODIFICATION: cysteine residue {C343} N-glycosylation site {N405} immunoglobulin superfamily domain {412-497} MOTIF: cysteine residue {C433} MODIFICATION: cysteine residue {C481} N-glycosylation site {N463} cysteine residue {C481} MODIFICATION: cysteine residue {C433} immunoglobulin superfamily domain {503-586} MOTIF: cysteine residue {C524} MODIFICATION: cysteine residue {C570} N-glycosylation site {N567} cysteine residue {C570} MODIFICATION: cysteine residue {C524} immunoglobulin superfamily domain {578-680} MOTIF: cysteine residue {C613} MODIFICATION: cysteine residue {C664} proteolytic site {621-622} N-glycosylation site {N646} cysteine residue {C664} MODIFICATION: cysteine residue {C613} transmembrane domain {705-725} CTNNB1 interaction {794-1070} MOTIF: kinase domain SITE: 796-1066 MOTIF: ATP-binding site NAME: ATP-binding site

Database Correlations

OMIM 601890 UniProt Q13308 PFAM correlations Entrez Gene 5754 Kegg hsa:5754

References

UniProt :accession Q13308