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trypsin-1; beta-trypsin; cationic trypsinogen; serine protease 1; trypsin I; contains: alpha-trypsin chain 1, alpha-trypsin chain 2 (PRSS1, TRP1, TRY1, TRYP1)
Function:
- has activity against the synthetic substrates
a) Boc-Phe-Ser-Arg-Mec
b) Boc-Leu-Thr-Arg-Mec
c) Boc-Gln-Ala-Arg-Mec
d) Boc-Val- Pro-Arg-Mec
- the single-chain form is more active than the two-chain form against all of these substrates
- preferential cleavage: Arg-|-Xaa, Lys-|-Xaa
- occurs in a single-chain form & a two-chain form, produced by proteolytic cleavage after Arg-122
Cofactor: binds 1 Ca+2 per subunit
Structure:
- belongs to the peptidase S1 family
- contains 1 peptidase S1 domain
Compartment:
- secreted, extracellular space
Pathology:
- mutations associated with hereditary chronic pancreatitis
Related
PRSS1 gene mutation
General
Ca+2 binding protein
secreted protein
trypsin
Properties
COMPARTMENT: extracellular compartment
SIZE: entity length = 247 aa
MW = 27 kD
MOTIF: signal sequence {1-15}
S1 domain {24-244}
MOTIF: cysteine residue {C30}
MODIFICATION: cysteine residue {C160}
cysteine residue {C48}
MODIFICATION: cysteine residue {C64}
histidine residue {H63}
cysteine residue {C64}
MODIFICATION: cysteine residue {C48}
Ca+2-binding site
SITE: 75-75
Ca+2-binding site
SITE: 77-77
Ca+2-binding site
SITE: 80-80
Ca+2-binding site
SITE: 85-85
aspartate residue {D107}
cysteine residue {C139}
MODIFICATION: cysteine residue {C206}
sulfotyrosine site {Y154}
cysteine residue {C160}
MODIFICATION: cysteine residue {C30}
cysteine residue {C171}
MODIFICATION: cysteine residue {C185}
cysteine residue {C185}
MODIFICATION: cysteine residue {C171}
aspartate residue {194}
cysteine residue {C196}
MODIFICATION: cysteine residue {C220}
serine residue {S200}
cysteine residue {C206}
MODIFICATION: cysteine residue {C139}
cysteine residue {C220}
MODIFICATION: cysteine residue {C196}
STATE: active state
Database Correlations
OMIM correlations
MORBIDMAP 276000
UniProt P07477
Pfam PF00089
Entrez Gene 5644
Kegg hsa:5644
ENZYME 3.4.21.4
References
- UniProt :accession P07477
- GeneReviews
http://www.ncbi.nlm.nih.gov/sites/genetests/lab/gene/PRSS1