tripartite motif-containing protein 28; transcription intermediary factor 1-beta; TIF1-beta; nuclear corepressor KAP-1; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; RING finger protein 96 (TRIM28, KAP1, RNF96, TIF1B)

Function: - forms a complex with a KRAB-domain transcription factor & increases the efficiency of KRAB-mediated repression - silences transcription through an interaction with HP1 proteins - acts as a corepressor of transcription for the KRAB Zn⁺² finger proteins & as a moderator of the repression activity - may play a role as a coactivator for CEBPB & NR3C1 in the transcriptional activation of the alpha-1-acid glycoprotein gene - phosphorylated upon DNA damage, probably by ATM or ATR - associated with HP1 alpha (CBX5), beta (CBX1) & gamma (CBX3) in interphase nuclei (putative) - interacts with ZNF382; enhances ZNF382 transcriptional repressor activity - interacts with CEBPB & NR3C1 (putative) - interacts with NCOR1 & CHD3 - interacts with SETDB1, ZFP53 & ZFP68 - interacts with CBX5 via the PxVxL motif - interacts with ZNF256 Structure: - the HP1 box is both necessary & sufficient for HP1 binding - the RING finger domain & the B-box domains mediate interaction with CEBPB - the PHD domain enhances the CEBPB transcriptional activity (putative) - contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif a) required for interaction with chromoshadow domains b) requires additional residues -7, -6, +4 & +5 of the central Val which contact the chromoshadow domain - belongs to the TRIM/RBCC family - contains 2 B box-type Zn⁺² fingers - contains 1 bromo domain - contains 1 PHD-type Zn⁺² finger - contains 1 RING-type Zn⁺² finger Compartment: - nucleus (putative) - associated with centromeric heterochromatin during cell differentiation through CBX1 (putative) Alternative splicing: named isoforms=2

General

nuclear protein phosphoprotein tripartite motif-containing protein (trim protein)

Properties

SIZE: entity length = 835 aa MW = 89 kD COMPARTMENT: cell nucleus MOTIF: alanine-rich region {2-58} MOTIF: alanine residue (SEVERAL) acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Ser phosphorylation site {S14} Ser phosphorylation site {S17} Ser phosphorylation site {S19} Ser phosphorylation site {S26} Ser phosphorylation site {S45} Ser phosphorylation site {S49} Ser phosphorylation site {S50} RING-finger {65-121} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif zinc finger B box SITE: 148-195 EFFECTOR-BOUND: Zn+2 zinc finger B box SITE: 204-245 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S258} Ser phosphorylation site {S440} Ser phosphorylation site {S466} Ser phosphorylation site {S471} Ser phosphorylation site {S473} HP1 box {476-513} MOTIF: Ser phosphorylation site {S479} PxVxL {481-494} Ser phosphorylation site {S489} Ser phosphorylation site {S501} alanine-rich region {526-530} MOTIF: alanine residue (SEVERAL) Thr phosphorylation site {T541} Ser phosphorylation site {S594} Ser phosphorylation site {S596} Ser phosphorylation site {S600} Ser phosphorylation site {S601} Zn finger PHD-type NAME: Zn finger PHD-type SITE: 625-672 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S681} Ser phosphorylation site {S683} Ser phosphorylation site {S689} bromodomain {697-801} MOTIF: Ser phosphorylation site {S752} Ser phosphorylation site {S757} Ser phosphorylation site {S824}

Database Correlations

OMIM 601742 UniProt Q13263 PFAM correlations Entrez Gene 10155 Kegg hsa:10155