transcription factor-like 5 protein (Cha transcription factor, HPV-16 E2-binding protein 1, E2BP-1, TCFL5, CHA, E2BP1)

Function: 1) transcription factor (putative) 2) isoform 1 may play a role in early spermatogenesis 3) efficient DNA binding requires dimerization with another bHLH protein Structure: - contains 1 basic helix-loop-helix (bHLH) domain Compartment: nucleus Alternative splicing: named isoforms=2 Expression: - isoform 1 is testis specific it is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids. - isoform 2 is pancreas specific Pathology: - antibodies against TCFL5 are present in sera from patients with Chagas' disease

General

DNA-binding protein transcription factor (TF)

Properties

SIZE: MW = 48 kD entity length = 452 aa COMPARTMENT: cell nucleus MOTIF: alanine-rich region {19-134} MOTIF: alanine residue (SEVERAL) R3 epitope (recognized by Chagas's antibodies) {299-308} DNA-binding motif SITE: 354-366 helix-loop-helix NAME: helix-loop-helix SITE: 367-402 FOR-BINDING-VIA: helix-loop-helix R1 epitope (recognized by Chagas's antibodies) {433-452}

Database Correlations

OMIM 604745 UniProt Q9UL49 Pfam PF00010

References

UniProt :accession Q9UL49