transcription factor IIIB 50 kD subunit (TFIIIB50, hTFIIIB50, B-related factor 2, BRF-2, hBRFU, BRF2, BRFU, PRO1470)

Function: - general activator of RNA polymerase 3 transcription factor exclusively required for RNA polymerase 3 transcription of genes with promoter elements upstream of initiation sites - component of TFIIIB complex - BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent - interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters - interacts with TBP & the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates RNA polymerase 3 transcription - forms a trimeric complex composed of TBP, BRF2 & mini-SNAPc complex (SNAP43, SNAP50, & the N-terminal 1/3 of SNAP190) on the promoter - assembly of the TBP-BRF2 complex is stimulated by SNAP190 - interacts with MAF1 & SNAPC4 Cofactor: binds 1 Zn⁺² per subunit (putative) Structure: - belongs to the TFIIB family - contains 1 TFIIB-type Zn⁺² finger Compartment: nucleus Expression: - down-regulated by epigallocatechin gallate (EGCG) treatment

General

nuclear protein protein subunit zinc finger protein

Properties

SIZE: entity length = 419 aa MW = 47 kD COMPARTMENT: cell nucleus MOTIF: Zinc finger NAME: Zinc finger SITE: 2-36 EFFECTOR-BOUND: Zn+2 MOTIF: Zn+2-binding site SITE: 7-7 Zn+2-binding site SITE: 10-10 Zn+2-binding site SITE: 28-28 Zn+2-binding site SITE: 31-31 consensus repeat {72-157} consensus repeat {173-249}

Database Correlations

OMIM 607013 UniProt Q9HAW0 Pfam PF08271 Kegg hsa:5529

References

UniProt :accession Q9HAW0