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transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1 (TGFA)

Function: - released by proteolysis of membrane-bound precursor - binds to EGF receptor - mitogen for mesenchymal cells - promotes angiogenesis - by itself, inhibits proliferation of most epithelial cells & lymphocytes - synergistic with TGF-beta in promoting anchorage- independent cell proliferation in soft agar - interacts with the PDZ domains of MAGI3, SDCBP & SNTA1 - interaction with SDCBP, is required for targeting to the cell surface - in the endoplasmic reticulum, in its immature form (i.e. with a prosegment & lacking full N-glycosylation), interacts with CNIH - in the Golgi apparatus, may form a complex with CNIH & GORASP2 - interacts (via cytoplasmic C-terminal domain) with NKD2 Structure: contains 1 EGF-like domain Compartment: - secreted, extracellular space - pro-TGF-alpha: - cell membrane; single-pass type 1 membrane protein Alternative splicing: named isoforms=5 Expression: - isoform 1, isoform 3 & isoform 4 are expressed in keratinocytes & tumor-derived cell lines

Interactions

molecular events

General

glycoprotein transforming growth factor (TGF)

Properties

SIZE: entity length = 160 aa MW = 17 kD COMPARTMENT: cellular membrane MOTIF: signal sequence {1-23} N-glycosylation site {N25} EGF domain {43-83} MOTIF: cysteine residue {C47} MODIFICATION: cysteine residue {C60} cysteine residue {C55} MODIFICATION: cysteine residue {C71} cysteine residue {C60} MODIFICATION: cysteine residue {C47} cysteine residue {C71} MODIFICATION: cysteine residue {C55} cysteine residue {C73} MODIFICATION: cysteine residue {C82} cysteine residue {C82} MODIFICATION: cysteine residue {C73} transmembrane domain {99-124} cysteine residue {C153} MODIFICATION: palmitate COMPARTMENT: membrane cysteine residue {C154} MODIFICATION: palmitate COMPARTMENT: membrane SECRETED-BY: macrophage

Database Correlations

OMIM 190170 UniProt P01135 Entrez Gene 7039 Kegg hsa:7039

References

  1. Primary Hyperlipoproteinemias, Steiner & Shafrir (eds), McGraw Hill, NY, 1991, pg 89
  2. Henderson B & Blake S Therapeutic potential of cytokine manipulation TIPS 13:145 1992 PMID: 1589908
  3. Entrez Gene :accession 7039
  4. UniProt :accession P01135