Contents

Search

transcription factor IIE (TFIIE)

Function: - initiates transcription in gene with RNA polymerase 2, TFIID (or Sp1?), & TFIIB bound - recruits TFIIH to the initiation complex & stimulates the RNA polymerase 2 C-terminal domain kinase & DNA-dependent ATPase activities of TFIIH [2] - both TFIIH & TFIIE are required for promoter clearance by RNA polymerase 2 - required for transcription Structure: - tetramer of 2 TFIIE-alpha & 2 TFIIE-beta chains [2] Genetics: - encoded by 2 genes

General

adenosine triphosphatase (ATPase) general transcription factor (basal transcription factor, transcription initiation factor) molecular complex

Properties

COMPARTMENT: cell nucleus MOTIF: active site MOTIF: adenine nucleotide-binding site SUBUNITS: general transcription factor IIE subunit 1 MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl HTH TFE/IIEalpha-type {14-104} Zn finger C4-type SITE: 129-157 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S268} acidic region {378-393} MOTIF: acidic residue (SEVERAL) transcription initiation factor IIE subunit beta MOTIF: serine-rich region {26-36} MOTIF: serine residue (SEVERAL) lysine-rich region {37-42} MOTIF: lysine residue (SEVERAL) DNA-binding motif SITE: 66-146 lysine-rich region {251-271} MOTIF: lysine residue (SEVERAL)

References

  1. Molecular Cell Biology (2nd ed) Darnell J; Lodish H & Baltimore D (eds), Scientific American Books, WH Freeman, NY 1990, pg 294
  2. Buratowski S. The basics of basal transcription by RNA polymerase II. Cell. 1994 Apr 8;77(1):1-3. Review. No abstract available. PMID: 8156586
  3. Drapkin R, Reinberg D. The multifunctional TFIIH complex and transcriptional control. Trends Biochem Sci. 1994 Nov;19(11):504-8. Review. PMID: 7855896
  4. UniProt :accession P29083

Component-of

transcriptional initiation complex

Components

general transcription factor IIE subunit 1; general transcription factor IIE 56 kD subunit; transcription initiation factor IIE subunit alpha; TFIIE-alpha; p56TFIIE (GTF2E1, TF2E1) transcription initiation factor IIE subunit beta; TFIIE-beta; general transcription factor IIE subunit 2; p34TFIIE (GTF2E2, TF2E2)