SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3; helicase-like transcription factor; sucrose nonfermenting protein 2-like 3; DNA-binding protein/plasminogen activator inhibitor 1 regulator; HIP116; RING finger protein 80 (HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1)

Function: - in contrast with other SMARC proteins, is does not seem to associate with actin or actin related proteins - instead, it may act as a sequence-specific DNA binding ATPase - has both helicase & E3 ubiquitin ligase activities - possesses intrinsic ATP-dependent nucleosome-remodeling activity - this activity may be required for transcriptional activation or repression of specific target promoters (putative) - these may include the SERPINE1 & HIV-1 promoters & the SV40 enhancer, to which this protein can bind directly - plays a role in error-free postreplication repair of damaged DNA & maintains genomic stability through acting as a ubiquitin ligase for Lys-63-linked polyubiquitination of chromatin-bound PCNA - protein modification; protein ubiquitination - binds to SP1 & SP3 independent of DNA - these interactions may be required for basal transcription of target genes - interacts with PCNA - the interaction promotes polyubiquitination of PCNA through association with the UBE2B-RAD18 & UBE2V2-UBE2N ubiquitin ligase complexes - interacts with RAD18, SHPRH, UBE2V2 & UBE2N Structure: - belongs to the SNF2/RAD54 helicase family, RAD16 subfamily - contains 1 helicase ATP-binding domain - contains 1 helicase C-terminal domain - contains 1 RING-type Zn⁺² finger Compartment: nucleus Alternative initiation: named isoforms=2 Expression: - expressed in brain, heart, kidney, liver, lung, pancreas, placenta & skeletal muscle Genetics: - subject to frequent epigenetic inactivation by promoter methylation in colon cancer

General

DNA-binding protein phosphoprotein ring finger protein SWI/SNF-related matrix-associated actin dependent regulator of chromatin subfamily A transcription factor (TF)

Properties

SIZE: entity length = 1009 aa MW = 114 kD COMPARTMENT: cell nucleus MOTIF: DNA-binding motif SITE: 38-287 ATP-binding site NAME: ATP-binding site SITE: 294-301 Thr phosphorylation site {T390} Tyr phosphorylation site {Y394} Ser phosphorylation site {S397} Ser phosphorylation site {S398} Ser phosphorylation site {S400} helicase NAME: helicase SITE: 435-606 MOTIF: DEAD/H box NAME: DEAD/H box SITE: 557-560 Ser phosphorylation site {S635} Thr phosphorylation site {T736} RING-finger {760-801} EFFECTOR-BOUND: Zn+2 FOR-BINDING-OF: DNA motif Helicase C-terminal {837-996} SP1/SP3 interaction {925-1009}

Database Correlations

OMIM 603257 UniProt Q14527 PFAM correlations Entrez Gene 6596 Kegg hsa:6596

References

UniProt :accession Q14527