surface plasmon resonance

No radioactive label. Smallest protein for which technique successfully employed 180,000 daltons (2003). ligand is attached to the sensor chip gold-dextran surface (maximum 400 runs on same surface) protein in sample binds to ligand changes in refractive index measured - resonance of electrons in gold excited by red light - ligand binding effect electron delocalization in some manner as to affect refractive index Gives on & off rates, kD

References

Biacore