bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; sulfurylase kinase 1; SK 1; SK1; includes: sulfate adenylyltransferase; ATP-sulfurylase; sulfate adenylate transferase; SAT; includes: adenylyl-sulfate kinase; 3'-phosphoadenosine-5'-phosphosulfate synthase; APS kinase; adenosine-5'-phosphosulfate 3'-phosphotransferase; adenylylsulfate 3'-phosphotransferase (PAPSS1, ATPSK1, PAPSS)

Function: - bifunctional enzyme with both ATP sulfurylase & APS kinase activity - mediates two steps in the sulfate activation pathway - the first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), - the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase) - in mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway - also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells - sulfur metabolism; sulfate assimilation ATP + sulfate = diphosphate + adenylyl sulfate ATP + adenylyl sulfate = ADP + 3'- phosphoadenylyl sulfate Inhibition: - inhibited by chlorate Structure: - in the N-terminal section; belongs to the APS kinase family - in the C-terminal section; belongs to the sulfate adenylyltransferase family Expression: - expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes & liver - also expressed in high endothelial venules (HEV) cells & in cartilage

General

kinase or ATP-phosphotransferase phosphoprotein

Properties

SIZE: entity length = 624 aa MW = 71 kD MOTIF: Adenylyl-sulfate kinase {1} ATP-binding site NAME: ATP-binding site SITE: 59-66 serine residue {S133} kinase domain MOTIF: ATP-binding site NAME: ATP-binding site phosphorylation site PP {521-525} Sulfate adenylyltransferase {624}

Database Correlations

OMIM 603262 UniProt O43252 PFAM correlations Entrez Gene 9061 Kegg hsa:9061 ENZYME correlations

References

UniProt :accession O43252