FACT complex subunit SSRP1; facilitates chromatin transcription complex subunit SSRP1; FACT 80 kD subunit; FACTp80; chromatin-specific transcription elongation factor 80 kD subunit; structure-specific recognition protein 1; hSSRP1; recombination signal sequence recognition protein 1; T160 (SSRP1, FACT80)

Function: - component of the FACT complex - also acts as a transcriptional coactivator for p63/TP63 - phosphorylated by CK2 following UV but not gamma irradiation; phosphorylation inhibits its DNA-binding activity - ubiquitinated; polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein - sumoylated - also component of a CK2-SPT16-SSRP1 complex - binds to histone H3-H4 tetramers, but not to intact nucleosomes - interacts with isoform gamma of p63/TP63 - interacts with SRF & NEK9 Structure: - the HMG box DNA-binding domain mediates DNA-binding - has both affinity & specificity for DNA damaged globally with cisplatin - belongs to the SSRP1 family - contains 1 HMG box DNA-binding domain Compartment: - nucleus - colocalizes with RNA polymerase 2 on chromatin - recruited to actively transcribed loci Pathology: - autoantibodies against SSRP1 are present in sera from patients with systemic lupus erythematosus, but not other rheumatic diseases

General

DNA-binding protein phosphoprotein

Properties

SIZE: entity length = 709 aa MW = 81 kD COMPARTMENT: cell nucleus MOTIF: acetylation site SITE: N-TERMINUS EFFECTOR-BOUND: acetyl Thr phosphorylation site {T170} Tyr phosphorylation site {Y311} Ser phosphorylation site {S437} acidic region {439-496} MOTIF: acidic residue (SEVERAL) Tyr phosphorylation site {Y441} Ser phosphorylation site {S444} peptide motif {450-451} serine-rich region {497-511} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S510} lysine-rich region {512-534} MOTIF: lysine residue (SEVERAL) HMG box SITE: 547-615 MOTIF: Tyr phosphorylation site {Y554} Ser phosphorylation site {S561} lysine-rich region {623-640} MOTIF: lysine residue (SEVERAL) serine-rich region {641-709} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S657} Ser phosphorylation site {S659} Ser phosphorylation site {S667} Ser phosphorylation site {S668} Ser phosphorylation site {S671} Ser phosphorylation site {S672} Ser phosphorylation site {S673} Ser phosphorylation site {S688}

Database Correlations

OMIM 604328 UniProt Q08945 PFAM correlations Entrez Gene 6749 Kegg hsa:6749