splicing factor 3B selections

splicing factor 3B (SF3B)

Function: - RNA splicing - excision of introns from pre-messenger RNA - component of the U2 snRNP & U11/U12 snRNP - SF3B associates with the splicing factor SF3A & a 12S RNA unit to form U2 snRNP - recognition of the pre-messenger RNA's branch site within the major & minor spliceosomes - required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA - sequence independent binding of SF3A/SF3B complex upstream of the branch site - may anchor U2 snRNP to the pre-mRNA - may also be involved in the assembly of the 'E' complex Subunits: - SF3B1/SAP155/SF3B155 - SF3B2/SAP145/SF3B155 - SF3B3/SAP130/SF3B130 - SF3B4/SAP49/SF3B49 - SF3B14A - PHF5A/SF3B14B - SF3B10 - SF3B125

General

molecular complex splicing factor; snRNP-associated protein

Properties

COMPARTMENT: cell nucleus MOTIF: RNA-binding motif FOR-BINDING-OF: ribonucleic acid SUBUNITS: splicing factor 3B subunit 1 COMPARTMENT: cytoplasm cell nucleus MOTIF: Thr phosphorylation site {T142} Thr phosphorylation site {T207} Thr phosphorylation site {T211} PPP1R8 interaction {223-491} MOTIF: Thr phosphorylation site {T223} Thr phosphorylation site {T227} Thr phosphorylation site {T235} Thr phosphorylation site {T244} Thr phosphorylation site {T248} Thr phosphorylation site {T267} Thr phosphorylation site {T296} Thr phosphorylation site {T299} Thr phosphorylation site {T313} Thr phosphorylation site {T316} Thr phosphorylation site {T326} Thr phosphorylation site {T328} Ser phosphorylation site {S344} Thr phosphorylation site {T350} Thr phosphorylation site {T354} HEAT repeat {529-568} HEAT repeat {569-603} HEAT repeat {604-641} HEAT repeat {643-677} HEAT repeat {680-718} HEAT repeat {763-801} HEAT repeat {843-881} HEAT repeat {1010-1048} HEAT repeat {1052-1090} HEAT repeat {1122-1160} HEAT repeat {1163-1201} splicing factor 3B subunit 2 COMPARTMENT: cell nucleus MOTIF: SAP {1-35} proline-rich region SITE: 68-73 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 81-90 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 106-109 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 226-230 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S266} glutamate-rich region {269-274} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S284} Ser phosphorylation site {S286} Thr phosphorylation site {T288} lysine-rich region {308-312} MOTIF: lysine residue (SEVERAL) Ser phosphorylation site {S320} Ser phosphorylation site {S337} Ser phosphorylation site {S339} Ser phosphorylation site {S393} Ser phosphorylation site {S408} Ser phosphorylation site {S412} Ser phosphorylation site {S413} glutamate-rich region {676-679} MOTIF: glutamate residue (SEVERAL) glutamate-rich region {697-703} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S755} Thr phosphorylation site {T757} splicing factor 3B subunit 3 COMPARTMENT: cell nucleus MOTIF: RNA-binding motif FOR-BINDING-OF: ribonucleic acid phosphorylation site splicing factor 3B subunit 4 COMPARTMENT: cell nucleus MOTIF: RNP motif NAME: RNP motif SITE: 13-91 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Tyr phosphorylation site {Y56} RNP motif NAME: RNP motif SITE: 100-179 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif proline-rich region SITE: 215-218 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 262-268 MOTIF: proline residue (SEVERAL) SF3B14A PHD finger-like domain protein 5A splicing factor 3B subunit 5 COMPARTMENT: cell nucleus MOTIF: Ser phosphorylation site {S9} ATP-dependent RNA helicase DDX42 COMPARTMENT: cell nucleus MOTIF: Thr phosphorylation site {T10} serine-rich region {43-46} MOTIF: serine residue (SEVERAL) Ser phosphorylation site {S96} Ser phosphorylation site {S109} Ser phosphorylation site {S111} coiled coil {116-157} glutamate-rich region {175-178} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S185} Q motif {253-281} helicase NAME: helicase SITE: 284-459 MOTIF: ATP-binding site NAME: ATP-binding site SITE: 297-304 DEAD/H box NAME: DEAD/H box SITE: 407-410 Helicase C-terminal {487-632} glycine-rich region {654-659} glycine-rich region {762-883} MOTIF: histidine-rich region {819-871} MOTIF: histidine residue (SEVERAL)

References

UniProt :accession Q86XP3
Golas MM, Sander B, Will CL, Luhrmann R, Stark H. Molecular architecture of the multiprotein splicing factor SF3b. Science. 2003 May 9;300(5621):980-4. PMID: 12738865

Component-of

U2-snRNP; U2 small nuclear ribonucleoprotein

Components

ATP-dependent RNA helicase DDX42 (DEAD box protein 42, splicing factor 3B-associated 125 kD protein, SF3b125, RNA helicase-related protein, RNAHP, RNA helicase-like protein, SF3b DEAD-box protein, RHELP, DDX42) splicing factor 3B subunit 1 (spliceosome associated protein 155, SAP 155, SF3b155, pre-mRNA splicing factor SF3b 155 kD subunit, SF3B1) splicing factor 3B subunit 2 (spliceosome associated protein 145, SAP 145, SF3b150, pre-mRNA splicing factor SF3b 145 kD subunit, SF3B2) splicing factor 3B subunit 3 (spliceosome associated protein 130, SAP 130, SF3b130, pre-mRNA splicing factor SF3b 130 kD subunit, SF3B4) splicing factor 3B subunit 4 (spliceosome associated protein 49, SAP 49, SF3b50, pre-mRNA splicing factor SF3b 49 kD subunit, STAF130, SF3B3, KIAA0017) splicing factor 3B subunit 5 (SF3b5, splicing factor SF3b 10 kD subunit, SF3B10)