splicing factor 3A selections

splicing factor 3A (SF3A)

Function: - RNA splicing - excision of introns from pre-messenger RNA - component of the U2 snRNP & U11/U12 snRNP - SF3A associates with the splicing factor SF3B & a 12S RNA unit to form U2 snRNP Subunits: - 3 subunits, 60, 66 & 120 kD

General

molecular complex splicing factor; snRNP-associated protein

Properties

COMPARTMENT: cell nucleus MOTIF: RNA-binding motif FOR-BINDING-OF: ribonucleic acid SUBUNITS: splicing factor 3A subunit 1 MOTIF: proline-rich region SITE: 10-16 MOTIF: proline residue (SEVERAL) SURP {52-94} glutamine-rich region {118-122} MOTIF: glutamine residue (SEVERAL) Thr phosphorylation site {T124} SURP {166-208} glutamate-rich region {260-267} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S320} Ser phosphorylation site {S329} Ser phosphorylation site {S359} proline-rich region SITE: 369-372 MOTIF: proline residue (SEVERAL) Tyr phosphorylation site {Y456} proline-rich region SITE: 557-560 MOTIF: proline residue (SEVERAL) proline-rich region SITE: 672-675 MOTIF: proline residue (SEVERAL) Ubiquitin-like {707-793} MOTIF: Tyr phosphorylation site {Y759} splicing factor 3A subunit 2 MOTIF: Zinc finger NAME: Zinc finger SITE: 54-84 EFFECTOR-BOUND: Zn+2 RNA-binding motif FOR-BINDING-OF: ribonucleic acid proline residue {233-236} (4) proline residue {252-256} (5) proline residue {458-462} (5) splicing factor 3A subunit 3 MOTIF: acetylation site SITE: 1-1 EFFECTOR-BOUND: acetyl Thr phosphorylation site {T277} glutamate-rich region {354-362} MOTIF: glutamate residue (SEVERAL) Ser phosphorylation site {S365} Ser phosphorylation site {S367} Ser phosphorylation site {S369} Zn finger matrin-type NAME: Zn finger matrin-type SITE: 406-437 EFFECTOR-BOUND: Zn+2 MOTIF: Tyr phosphorylation site {Y414}

References

Kramer A, Ferfoglia F, Huang CJ, Mulhaupt F, Nesic D, Tanackovic G. Structure-function analysis of the U2 snRNP-associated splicing factor SF3a. Biochem Soc Trans. 2005 Jun;33(Pt 3):439-42. Review. PMID: 15916536
Tanackovic G, Kramer A. Human splicing factor SF3a, but not SF1, is essential for pre-mRNA splicing in vivo. Mol Biol Cell. 2005 Mar;16(3):1366-77. Epub 2005 Jan 12. PMID: 15647371

Component-of

U2-snRNP; U2 small nuclear ribonucleoprotein

Components

splicing factor 3A subunit 1 (spliceosome associated protein 114, SAP 114, SF3a120, SF3A1) splicing factor 3A subunit 2 (spliceosome associated protein 62, SAP 62, SF3a66, SF3A2) splicing factor 3A subunit 3 (spliceosome associated protein 61, SAP 61, SF3a60, SF3A3)