spermine oxidase; polyamine oxidase 1; PAO-1; PAOh1 (SMOX, C20orf16, SMO, UNQ3039/PRO9854)

Function: 1) amine & polyamine degradation; spermine degradation 2) flavoenzyme 3) catalyzes oxidation of spermine to spermidine 4) can also use N(1)-acetylspermine & spermidine as substrates 5) regulation of polyamine intracellular concentration 6) determinant of cellular sensitivity to antitumor polyamine analogs (putative) 7) beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal 8) inhibited at more than 90% by SL-11144, SL-11150 & SL-11158, at concentrations less than 1uM 9) low affinity for acetylated polyamine Cofactor: FAD, noncovalently linked Kinetic parameters: - KM=0.6 uM for spermine (isoform 1) - KM=0.5 uM for spermine (isoform 6) - KM=3.0 uM for N1-acetylspermine (isoform 1 & isoform 6) - Kcat is 27-fold higher with spermine than N1-acetylspermine as substrate for isoform 1 & isoform 6 - Optimum pH is 9.5 with spermine as substrate; Structure: belongs to the flavin monoamine oxidase family Compartment: cytoplasm, nucleus Alternative splicing: named isoforms=5 - at least 5 different isozymes are produced by alternative splicing of 8 exons - the resultant proteins have different biochemical characteristics & substrate specificity Expression: - widely expressed - induction by antitumor polyamine analogs

General

flavoprotein oxidoreductase

Properties

SIZE: entity length = 555 aa MW = 62 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: active site

Database Correlations

UniProt Q9NWM0 Pfam PF01593 Entrez Gene 54498 Kegg hsa:54498 ENZYME 1.5.3.16

References

UniProt :accession Q9NWM0