sororin; cell division cycle-associated protein 5; p35 (CDCA5)

Function: - regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin - may antagonize the action of WAPAL which stimulates cohesin dissociation from chromatin - cohesion ensures that chromosome partitioning is accurate in both meiotic & mitotic cells & plays a role in DNA repair - required for efficient DNA double-stranded break repair - phosphorylation, as cells enter mitosis, disrupts the interaction with PDS5A & relieves the inhibition of WAPAL by CDCA5 - ubiquitinated by the APC/C complex in G1 phase, leading to its degradation (probable) - interacts with the chromatin-bound cohesin complex - interaction is indirect - occurs after DNA replication - requires acetylation of the cohesin component SMC3 - interacts (via the FGF motif) with PDS5A & PDS5B - interaction prevents interaction of PDS5A with WAPAL Structure: - the KEN box is required for the association with the APC/C complex (putative) - belongs to the sororin family Compartment: - nucleus, chromosome, cytoplasm - associates with nuclear chromatin from S phase until metaphase & is released in the cytoplasm upon nuclear envelope breakdown Notes: - named sororin after the latin word 'soror', which means 'sister', because of its critical role in sister chromatid cohesion

General

nuclear protein phosphoprotein

Properties

SIZE: entity length = 252 aa MW = 28 kD COMPARTMENT: cytoplasm nuclear membrane MOTIF: Ser phosphorylation site {S29} Ser phosphorylation site {S33} Ser phosphorylation site {S35} Thr phosphorylation site {T48} Ser phosphorylation site {S50} Ser phosphorylation site {S75} Ser phosphorylation site {S79} Ser phosphorylation site {S83} KEN box {88-90} Thr phosphorylation site {T105} Ser phosphorylation site {S107} Thr phosphorylation site {T111} Thr phosphorylation site {T115} Thr phosphorylation site {T159} FGF {166-168} Ser phosphorylation site {S209}

Database Correlations

OMIM 609374 UniProt Q96FF9 Pfam PF09666 Entrez Gene 113130 Kegg hsa:113130

References

UniProt :accession Q96FF9