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SH3 adapter protein SPIN90 (NCK-interacting protein with SH3 domain, SH3 protein interacting with Nck 90 kD, VacA-interacting protein 54 kD, VIP54, AF3p21, diaphanous protein-interacting protein, Dia-interacting protein 1, DIP-1, NCKIPSD, AF3P21, SPIN90)
Function:
- role in stress fiber formation induced by active DRF1
- induces microspike formation, in vivo
- in vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42
- role in maintenance of sarcomeres &/or in the assembly of myofibrils into sarcomeres
- implicated in regulation of actin polymerization & cell adhesion
- associates with intermediate filaments, vimentin & desmin
- binds 1st & 3rd SH3 domains of NCK
- binds proline-rich domains of N-WASP via its SH3 domain
- binds DRF1
- binds SH3 domains of GRB2 via its proline-rich domains
Structure: contains 1 SH3 domain
Compartment:
- nucleus
- colocalizes with DRF1 at membrane ruffles, & with Nck at Z-disks in mature cardiac myocytes
Alternative splicing: named isoforms=2
Expression:
- expressed in heart, brain, skeletal muscle, kidney, liver > placenta, lung, small intestine, leukocytes > colon, thymus, spleen.
Pathology:
- chromosomal translocation t(3;11)(p21;q23) involving NCKIPSD with MLL is found in therapy-related leukemia
General
nuclear protein
Properties
SIZE: MW = 79 kD
entity length = 722 aa
COMPARTMENT: cell nucleus
MOTIF: src homology 3 [SH3] domain
SITE: 1-58
MOTIF: Breakpoint for translocation to form MLL- AF3P21 oncogene {57-58}
proline-rich region
SITE: 171-275
MOTIF: proline residue (SEVERAL)
nuclear translocation signal {175-192}
threonine-rich region {197-240}
MOTIF: threonine residue (SEVERAL)
Database Correlations
OMIM 606671
UniProt Q9NZQ3
Pfam PF00018
References
- UniProt :accession Q9NZQ3
- Atlas of Genetics and Cytogenetics in Oncology and Haematology
http://atlasgeneticsoncology.org/Genes/AF3p21ID228.html