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serum response factor; SRF (SRF)
Function:
- transcription factor & DNA-binding protein
- binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS)
- required for cardiac differentation & maturation
- binds DNA as a multimer, probably a dimer
- interacts with MLLT7/FOXO4, NKX3A & SSRP1
- interacts with ARID2 & SRFBP1
- forms complexes with ARID2, MYOCD, NKX2-5 & SRFBP1
- SRF binding to RAP74 may be important for SRF-activated transcription [4]
- phosphorylated by PRKDC
Structure: contains 1 MADS-box domain
Compartment: nucleus
Interactions
molecular events
General
DNA-binding protein
oligomerizing protein
phosphoprotein
transcription factor (TF)
Properties
SIZE: entity length = 508 aa
MW = 52 kD
COMPARTMENT: cell nucleus
MOTIF: glycine-rich region {13-142}
MOTIF: Tyr phosphorylation site {Y76}
Ser phosphorylation site {S77}
Ser phosphorylation site {S79}
acidic region {80-90}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S83}
Ser phosphorylation site {S85}
Ser phosphorylation site {S103}
DNA-binding motif
SITE: 133-222
MOTIF: MADS domain or MADS box {141-201}
MOTIF: DNA-binding motif
binding site
FOR-BINDING-OF: transcription factor
dimerization {168-222}
Ser phosphorylation site {S224}
acidic region {242-258}
MOTIF: acidic residue (SEVERAL)
Ser phosphorylation site {S253}
Ser glycosylation site {S277}
Ser glycosylation site {S307}
Ser glycosylation site {S309}
Ser glycosylation site {S316}
Ser glycosylation site {S383}
Ser phosphorylation site {S435}
Ser phosphorylation site {S446}
Database Correlations
OMIM 600589
UniProt P11831
Pfam PF00319
Entrez Gene 6722
Kegg hsa:6722
References
- Alberts et al Molecular Biology of the Cell, 2nd ed.
1989, pg 566
- UniProt :accession P11831
- Berk AJ.
Regulation of eukaryotic transcription factors by post-
translational modification.
Biochim Biophys Acta. 1989 Nov 2;1009(2):103-9. Review.
PMID: 2529903
- Joliot V, Demma M, Prywes R.
Interaction with RAP74 subunit of TFIIF is required for
transcriptional activation by serum response factor.
Nature. 1995 Feb 16;373(6515):632-5.
PMID: 7854423