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serine/threonine protein kinase tousled-like 2; PKU-alpha; Tousled-like kinase 2 (TLK2)
Function:
- rapidly & transiently inhibited by phosphorylation following generation of DNA double-stranded breaks during S-phase
- this is cell cycle checkpoint & ATM-pathway dependent & appears to regulate processes involved in chromatin assembly
- cell-cycle regulated, maximal activity in S-phase
- inactivated by phosphorylation at Ser-750, potentially by CHK1
- heterodimerizes with TLK1
- interacts with ASF1A & ASF1B
Cofactor: Mg+2
Structure:
- belongs to the protein kinase superfamily, Ser/Thr protein kinase family
- contains 1 protein kinase domain
Compartment: nucleus
Alternative splicing: named isoforms=3
Expression:
- widely expressed. present in fetal placenta, liver, kidney, pancreas, heart & skeletal muscle
- also found in adult cell lines
General
nuclear protein
serine/threonine kinase
Properties
SIZE: entity length = 772 aa
MW = 88 kD
COMPARTMENT: cell nucleus
MOTIF: Ser phosphorylation site {S32}
Tyr phosphorylation site {Y70}
Thr phosphorylation site {T72}
Ser phosphorylation site {S73}
Ser phosphorylation site {S94}
Thr phosphorylation site {T98}
Ser phosphorylation site {S99}
Ser phosphorylation site {S110}
Ser phosphorylation site {S111}
Ser phosphorylation site {S115}
Ser phosphorylation site {S117}
Ser phosphorylation site {S134}
Ser phosphorylation site {S196}
coiled coil {225-276}
Thr phosphorylation site {T300}
coiled coil {317-347}
coiled coil {403-451}
kinase domain
SITE: 462-741
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 468-476
ATP-binding site
NAME: ATP-binding site
SITE: 491-491
aspartate residue {D592}
Ser phosphorylation site {S750}
Ser phosphorylation site {S753}
Ser phosphorylation site {S769}
Ser phosphorylation site {S770}
Ser phosphorylation site {S771}
STATE: active state
Database Correlations
OMIM 608439
UniProt Q86UE8
Pfam PF00069
Entrez Gene 11011
Kegg hsa:11011
ENZYME 2.7.11.1
References
UniProt :accession Q86UE8