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serine/threonine protein kinase Nek7; never in mitosis A-related kinase 7; NimA-related protein kinase 7 (NEK7)
Function:
- serine/threonine protein kinase
- role in mitotic cell cycle progression
- required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation & cytokinesis
- phosphorylates RPS6KB1
- binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97
- phosphorylation at Ser-195 required for its activation
- interacts with NEK9
Cofactor: Mg+2
Structure:
- monomer
- displays an autoinhibited conformation:
- Tyr-97 side chain points into the active site, interacts with the activation loop, & blocks the alphaC helix
- the autoinhibitory conformation is released upon binding with NEK9
- the NTE (N-terminal extension) motif is a structural component of the catalytic domain & thus contributes to activity
- belongs to the protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
- contains 1 protein kinase domain
Compartment:
- nucleus, cytoplasm
- cytoskeleton, spindle pole, centrosome
- present at centrosome throughout the cell cycle
- also detected at spindle midzone of the anaphase cells & eventually concentrates at the midbody
Alternative splicing: named isoforms=2
Expression:
- highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte & spleen
- lower expression in ovary, prostate & kidney
- not expressed in small intestine
General
nuclear protein
serine/threonine protein kinase NEK (never in mitosis gene A-related kinase, NimA-related protein kinase, NimA-like protein kinase)
Properties
SIZE: entity length = 302 aa
MW = 35 kD
COMPARTMENT: cytoplasm
cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
NTE motif {20-33}
kinase domain
SITE: 34-299
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 40-48
ATP-binding site
NAME: ATP-binding site
SITE: 63-63
tyrosine residue {97}
aspartate residue {D161}
Ser phosphorylation site {S187}
Ser phosphorylation site {S188}
Thr phosphorylation site {T190}
Ser phosphorylation site {S195}
Ser phosphorylation site {S204}
STATE: active state
Database Correlations
OMIM 606848
UniProt Q8TDX7
Pfam PF00069
Entrez Gene 140609
Kegg hsa:140609
ENZYME 2.7.11.
References
UniProt :accession Q8TDX7