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serine/threonine protein kinase MRCK beta; CDC42-binding protein kinase beta; myotonic dystrophy kinase-related CDC42-binding kinase beta; myotonic dystrophy protein kinase-like beta; MRCK beta; DMPK-like beta (CDC42BPB, KIAA1124)
Function:
- downstream effector of CDC42 in cytoskeletal reorganization
- regulates actomyosin contractility required for cell invasion, via regulation of MYPT1 & thus MLC2 phosphorylation
- phorbol ester activates resulting in dimerization via N-terminal region & kinase activation by trans- autophosphorylation
- interacts tightly with GTP-bound but not GDP-bound CDC42
Structure:
- homodimer & homotetramer via the coiled coil regions
- maintained in an inactive, closed conformation by an interaction between the kinase domain & negative autoregulatory C-terminal coiled-coil
- contains 1 AGC-kinase C-terminal domain
- contains 1 CNH domain
- contains 1 CRIB domain
- contains 1 PH domain
- contains 1 phorbol-ester/DAG-type Zn+2 finger
- contains 1 protein kinase domain
Compartment:
- cytoplasmic, with dispersed punctate distribution, concentrating along the cell periphery, especially at the leading edge & cell-cell junction
Expression: ubiquitously expressed
General
oligomerizing protein
serine/threonine kinase
zinc finger protein
Properties
SIZE: entity length = 1711 aa
MW = 194 kD
COMPARTMENT: cytoplasm
MOTIF: kinase domain
SITE: 76-342
MOTIF: ATP-binding site
NAME: ATP-binding site
SITE: 82-90
ATP-binding site
NAME: ATP-binding site
SITE: 105-105
aspartate residue {D200}
Ser phosphorylation site {S221}
Ser phosphorylation site {S233}
Thr phosphorylation site {T239}
AGC-kinase C-terminal {343-413}
MOTIF: Ser phosphorylation site {S362}
Ser phosphorylation site {S363}
coiled coil {431-815}
MOTIF: Thr phosphorylation site {T676}
coiled coil {878-939}
MOTIF: Ser phosphorylation site {S914}
Tyr phosphorylation site {Y954}
Zinc finger
NAME: Zinc finger
SITE: 1025-1075
EFFECTOR-BOUND: Zn+2
PH domain {1095-1214}
MOTIF: Thr phosphorylation site {T1196}
CNH {1240-1513}
CRIB domain {1583-1596}
Ser phosphorylation site {S1686}
Ser phosphorylation site {S1690}
Ser phosphorylation site {S1693}
STATE: active state
Database Correlations
OMIM 614062
UniProt Q9Y5S2
PFAM correlations
Entrez Gene 9578
Kegg hsa:9578
ENZYME 2.7.11.
References
- UniProt :accession Q9Y5S2
- NIEHS-SNPs
http://egp.gs.washington.edu/data/cdc42bpb/