seprase; 170 kD melanoma membrane-bound gelatinase; fibroblast activation protein alpha; integral membrane serine protease (FAP)

Function: - in association with DPP4, involved in the pericellular proteolysis of extracellular matrix, the migration & invasion of endothelial cells into the extracellular matrix - may have a role in tissue remodeling during development & wound healing - degrades gelatin & heat-denatured type collagen type 1 & collagen type 4, but not native collagen type 1 or native collagen type 4 - does not cleave laminin, fibronectin, fibrin or casein - homodimer, or heterodimer with DPP4 - the monomer is inactive Structure: - N-glycosylated - the N-terminus may be blocked - belongs to the peptidase S9B family Compartment: - cell membrane, cell projection, lamellipodium membrane - single-pass type 2 membrane protein - found in cell surface lamellipodia, & on shed vesicles - colocalized with DPP4 in lamellipodia of migratory activated endothelial cells in collagenous matrix Alternative splicing: named isoforms=2 Note: major isoform Expression: - in fibroblasts at times & sites of tissue remodeling during development, tissue repair, & carcinogenesis - endothelial cells during angiogenesis ? Pathology: - may contribute to invasiveness in malignant cancers - found in invadopodia, colocalized with DPP4 - colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma

General

glycoprotein membrane protein serine protease

Properties

SIZE: entity length = 760 aa MW = 88 kD COMPARTMENT: plasma membrane MOTIF: transmembrane domain {5-25} N-glycosylation site {N49} N-glycosylation site {N92} N-glycosylation site {N99} binding site SITE: 203-203 FOR-BINDING-OF: Substrate binding site SITE: 204-204 FOR-BINDING-OF: Substrate N-glycosylation site {N227} N-glycosylation site {N314} cysteine residue {C321} MODIFICATION: cysteine residue {C332} cysteine residue {C332} MODIFICATION: cysteine residue {C321} cysteine residue {C438} MODIFICATION: cysteine residue {C441} cysteine residue {C441} MODIFICATION: cysteine residue {C438} cysteine residue {C448} MODIFICATION: cysteine residue {C466} cysteine residue {C466} MODIFICATION: cysteine residue {C448} serine residue {S624} cysteine residue {C643} MODIFICATION: cysteine residue {C755} N-glycosylation site {N679} aspartate residue {D702} histidine residue {H734} cysteine residue {C755} MODIFICATION: cysteine residue {C643}

Database Correlations

OMIM 600403 UniProt Q12884 PFAM correlations Entrez Gene 2191 Kegg hsa:2191

References

UniProt :accession Q12884