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sepiapterin reductase; 7,8-dihydrobiopterin:NADP+oxidoreductase (SPR)
Function:
- catalyzes final one or two reductions in tetrahydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin
- in vitro phosphorylation of Ser-213 by CaMK2 does not change kinetic parameters
7,8-dihydrobiopterin + NADP+ sepiapterin + NADPH
tetrahydrobiopterin + 2 NADP+
6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH
Kinetic parameters:
- KM=14.3 uM for sepiapterin
- KM=10 uM for NADPH
Structure:
- homodimer
- belongs to the sepiapterin reductase family
Compartment: cytoplasm
Pathology:
- depressed synthesis of tetrahydrobiopterin may play a role in a variety of human diseases
General
oxidoreductase
phosphoprotein
Properties
SIZE: entity length = 261 aa
MW = 28 kD
COMPARTMENT: cytoplasm
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
cofactor-binding site [13-39]
COFACTOR-BOUND: NADP
MOTIF: Pterin binding {28-32}
Ser phosphorylation site {S213}
Database Correlations
OMIM 182125
MORBIDMAP 182125
UniProt P35270
Pfam PF00106
Entrez Gene 6697
Kegg hsa:6697
ENZYME 1.1.1.153
References
- UniProt :accession P35270
- GeneReviews
https://www.genecards.org/cgi-bin/carddisp.pl?gene=SPR