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scaffold attachment factor B1; SAF-B; SAF-B1; HSP27 estrogen response element-TATA box-binding protein; HSP27 ERE-TATA-binding protein (SAFB, HAP, HET, SAFB1)
Function:
- binds to scaffold/matrix attachment region (S/MAR) DNA & forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins & RNA polymerase 2) coupling transcription & RNA processing
- can function as an estrogen receptor corepressor
- can also bind to the HSP27 promoter & decrease its transcription
- when associated with RBMX, binds to & stimulates transcription from the SREBF1 promoter (putative)
- can inhibit cell proliferation
- sumoylated by PIAS1 with SUMO1 & SUMO2/3
- desumoylated by SENP1
- sumoylation is required for transcriptional repressor activity
- interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 & TRA2B/SFRS10 (putative)
- interacts with isoform 1 & isoform 2 of SRPK1 & inhibits its activity
- interacts with RBMX (putative)
Structure:
- monomer; can form homodimers
- contains 1 RRM domain (RNA recognition motif)
- contains 1 SAP domain
Compartment: nucleus
Expression:
- ubiquitous
- expressed at high levels in the CNS
- expressed at low levels in the liver
- expressed in a wide number of breast cancer cell lines
General
nuclear protein
phosphoprotein
RNA-binding protein
scaffold protein
Properties
SIZE: entity length = 915 aa
MW = 103 kD
COMPARTMENT: cell nucleus
MOTIF: acetylation site
SITE: N-TERMINUS
EFFECTOR-BOUND: acetyl
SAP {31-65}
MOTIF: Ser phosphorylation site {S55}
Thr phosphorylation site {T194}
Ser phosphorylation site {S235}
Ser phosphorylation site {S247}
Ser phosphorylation site {S288}
Ser phosphorylation site {S344}
Ser phosphorylation site {S373}
Ser phosphorylation site {S383}
Ser phosphorylation site {S384}
RNP motif
NAME: RNP motif
SITE: 406-484
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
NAME: ribonucleoprotein-1 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
ribonucleoprotein-2 motif
FOR-BINDING-OF: ribonucleic acid
MOTIF: ribonucleoprotein-1 motif
ribonucleoprotein-2 motif
POLR2A interaction {528-792}
MOTIF: Ser phosphorylation site {S555}
Ser phosphorylation site {S557}
Ser phosphorylation site {S589}
SAFB2 interaction {599-915}
MOTIF: nuclear translocation signal {599-616}
Ser phosphorylation site {S601}
Ser phosphorylation site {S604}
arginine-rich region {612-831}
MOTIF: arginine residue (SEVERAL)
glutamate-rich region {625-705}
MOTIF: glutamate residue (SEVERAL)
glycine-rich region {785-903}
Ser phosphorylation site {S808}
Database Correlations
OMIM 602895
UniProt Q15424
Pfam PF02037
Entrez Gene 6294
Kegg hsa:6294
References
UniProt :accession Q15424