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rRT-PCR Swine Flu Panel; influenza A H1N1 rRT-PCR
Indications:
- swine flu
Clinical significance:
- for use with viral culture
Specimen: nasopharyngeal &/or nasal swab specimens
Procedure:
- Swine Influenza Virus Real-time RT-PCR Detection Panel
- panel of oligonucleotide primers & dual-labeled hydrolysis (Taqman) probes for use in the real-time RT-PCR assay on the ABI 7500 FastDx Real-Time PCR instrument
- in vitro qualitative detection of swine influenza viral RNA in nasopharyngeal &/or nasal swab specimens
- universal swine influenza swInfA (NP gene) & swH1 (HA gene) primer & probe sets are designed for detection of swine A/H1N1 influenza virus
Includes the following primer & probe sets:
- InfA detects universal influenza A strains
- swInfA specifically detects swine influenza A strains (NP gene)
- sw H1 is specific for swine influenza A, subtype H1 (HA gene)
Includes control materials:
- RNase P detects human RNase P; used as a positive control to indicate that adequate isolation of nucleic acid resulted from the extraction of the clinical specimen
- Swine Influenza Positive Control (SIPC) is a positive control designed to react with all the primer & probe sets including RNase P
General
infectious agent nucleic acid
microbiology panel
References
- Letter of Authorization:
FDA: Food and Drug Administration
Emergency Use of the Swine Influenza Virus Real-time RT-PCR
Detection Panel
http://www.fda.gov/cdrh/emergency/panel-authorization.html
- Fact Sheet for Healthcare Providers:
Interpreting Swine Influenca RT-PCR Detection Panel Test
Results
FDA: Food and Drug Administration
http://www.fda.gov/cdrh/emergency/panel-factsheet1.html
- Centers for Disease Control (CDC)
Use of Rapid Influenza Diagnostic Tests for Patients with
Influenza-like Illness during the Novel H1N1 Influenza Virus
(Swine Flu) Outbreak
Information for Health Care Professionals
May 2, 2009
http://www.cdc.gov/h1n1flu/guidance/rapid_testing.htm
- H1N1 by PCR
Laboratory Test Directory ARUP: 2004217