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RNA polymerase-2 yeast holoenzyme

A subcomplex that associates with RNA polymerase 2, TFIIH, & TFIIB to form the pol II holoenzyme in yeast.

General

fungus-specific molecule RNA polymerase-2

Properties

COMPARTMENT: cell nucleus CELL: Saccharomyces cerevisiae KINGDOM: plant DIVISION: EUMYCOPHYTA SUBUNITS: RNA polymerase-2 L' MOTIF: Zn finger C2H2-type SITE: 71-87 EFFECTOR-BOUND: Zn+2 MOTIF: Zn+2-binding site SITE: 71-71 Zn+2-binding site SITE: 74-74 Zn+2-binding site SITE: 81-81 Zn+2-binding site SITE: 84-84 Zn+2-binding site SITE: 111-111 Zn+2-binding site SITE: 114-114 Tyr phosphorylation site {Y145} Zn+2-binding site SITE: 154-154 Zn+2-binding site SITE: 184-184 Mg+2-binding site SITE: 495-495 Mg+2-binding site SITE: 497-497 Mg+2-binding site SITE: 499-499 Bridging helix {833-845} repeat {1593-1960} MOTIF: consensus repeat {1593-1960} SEQUENCE-IS: Y-[ST]-P-[STQ]-[ST]-P-[SRTEVKGN] Ser phosphorylation site {S1878} Tyr phosphorylation site {Y1923} Ser phosphorylation site {S1927} Thr phosphorylation site {T1933} Ser phosphorylation site {S1934} RNA polymerase-2 L MOTIF: Mg+2-binding site SITE: 792-792 Zn finger C4-type SITE: 1119-1140 EFFECTOR-BOUND: Zn+2 MOTIF: Zn+2-binding site SITE: 1119-1119 Zn+2-binding site SITE: 1122-1122 Zn+2-binding site SITE: 1137-1137 Zn+2-binding site SITE: 1140-1140 SRB subcomplex transcription factor IIH transcription factor IIB MOTIF: Zinc finger NAME: Zinc finger SITE: 11-42 EFFECTOR-BOUND: Zn+2 MOTIF: Zn+2-binding site SITE: 15-15 Zn+2-binding site SITE: 18-18 Zn+2-binding site SITE: 34-34 Zn+2-binding site SITE: 37-37 consensus repeat {124-200} consensus repeat {218-294}

References

Koleske et al TIBS 20:113 1995

Components

RNA polymerase 2 140 kD subunit (RPB2, RNA polymerase 2 subunit B, RNA polymerase 2 subunit 2, POLR2B) RNA polymerase 2 largest subunit; RPB1; RNA polymerase 2 subunit A; RNA polymerase 2 subunit B1 (POLR2A) SRB subcomplex transcription factor IIB; general transcription factor; S300-II (GTF2B, TF2B, TFIIB) transcription factor IIH (TFIIH) or BTF2