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RNA polymerase 1-associated factor PAF49 (anti-sense to ERCC-1 protein, ASE-1, CD3-epsilon-associated protein, CD3E-associated protein, CAST, CD3EAP, ASE1, CAST, PAF49)
Function:
1) initiation of rRNA transcription by RNA polymerase 1 holoenzyme
2) isoform 2 described as a component of T-cell receptor complex*
- may undergoes TYR phosphorylation upon TCR stimulation
3) interacts with TAFI48 subunit of SL1 complex, PRAF1
4) isoform 2 interacts with CD3E
Compartment:
- nucleus
- found at the fibrillar centers of nucleolus in interphase
- localized to nucleolus organizer regions of the chromosomes during cell division
Alternative splicing: named isoforms=2
Genetics:
- gene is in an antisense orientation to & overlaps the gene of DNA repair enzyme ERCC1; this gene overlap is conserved in mouse suggesting an important biologic function
* evidence favors rRNA transcription rather than T-cell receptor signaling
General
nuclear protein
Properties
SIZE: MW = 55 kD
entity length = 510 aa
COMPARTMENT: cell nucleus
MOTIF: lysine-rich region {174-177}
MOTIF: lysine residue (SEVERAL)
lysine-rich region {212-217}
MOTIF: lysine residue (SEVERAL)
lysine-rich region {422-428}
MOTIF: lysine residue (SEVERAL)
glutamine-rich region {504-508}
MOTIF: glutamine residue (SEVERAL)
Database Correlations
OMIM 107325
UniProt O15446
References
UniProt :accession O15446