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RNA interference; clustered regularly interspaced short palindromic repeats (CRISPR)
Indications:
- useful for identifying molecular targets for treatment of disease [1]
Principle:
- siRNA may be used to selectively turn off translation of specific proteins
Clinical significance:
- used to identify proteins responsible for resistance to monoclonal antibody therapy for melanoma
Notes:
- CRISPR may play role in RNA interference
Related
CRISPR; clustered regularly interspaced short palindromic repeats
gene editing
short interfering double-stranded RNA; antisense oligonucleotide (siRNA, microRNA, miRNA)
General
biochemistry
molecular diagnostic test
References
- Wang T et al.
Genetic screens in human cells using the CRISPR-Cas9 system.
Science 2014 Jan 3; 343:80
PMID: 24336569
http://www.sciencemag.org/content/343/6166/80
- Shalem O et al.
Genome-scale CRISPR-Cas9 knockout screening in human cells.
Science 2014 Jan 3; 343:84.
PMID: 24336571
http://www.sciencemag.org/content/343/6166/84
- Niu Y et al.
Generation of gene-modified cynomolgus monkey via Cas9/RNA-
mediated gene targeting in one-cell embryos.
Cell 2014 Jan 30;
PMID: 24486104
http://www.cell.com/retrieve/pii/S0092867414000798
- Pennisi E.
Transgenic animals. Editing of targeted genes proved possible
in monkeys.
Science 2014 Jan 31; 343:476
PMID: 24482459