RNA-binding protein with serine-rich domain 1; SR-related protein LDC2 (RNPS1, LDC2)

Function: - component of pre- & post-splicing multiprotein mRNP complexes - enhances the formation of the ATP-dependent A complex of spliceosome - involved in both constitutive splicing & with SRP54 & SFRS10 - modulation of alternative splicing in a substrate-dependent manner - participates in mRNA 3'end cleavage - involved in RENT2-dependent nonsense-mediated decay of mRNAs containing premature stop codons - mediates increase of mRNA abundance & translational efficiency - binds spliced mRNA 20-25 nt upstream of exon-exon junctions - component of the active spliceosome. - associates with polysomes - found in a mRNA splicing-dependent exon junction complex - found in a post-splicing complex - phosphorylated on one or more of 4 Ser/Thr (S43, T49, S52, S53) - S53 phosphorylation stimulates splicing activity in vitro - interacts with the cleaved p110 isoform of CDC2L1, CSNK2A1, PNN, SART3, SRP54, SRRM1 & TRA2B/SFRS10 Structure: - belongs to the splicing factor SR family - contains 1 RRM domain (RNA recognition motif Compartment: - nuclear speckles & cytoplasmic - nucleocytoplasmic shuttling Expression: ubiquitous

General

nuclear protein phosphoprotein

Properties

SIZE: entity length = 305 aa MW = 34 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: domain {1-220} MOTIF: domain {1-161} lysine-rich region {7-64} MOTIF: lysine residue (SEVERAL) Ser phosphorylation site {S27} Ser phosphorylation site {S53} serine-rich region {67-141} MOTIF: serine residue (SEVERAL) domain {69-121} Ser phosphorylation site {S125} arginine-rich region {128-154} MOTIF: arginine residue (SEVERAL) Ser phosphorylation site {S155} domain {156-242} MOTIF: Ser phosphorylation site {S157} RNP motif NAME: RNP motif SITE: 161-240 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Tyr phosphorylation site {Y205} domain {238-305} MOTIF: proline-rich region SITE: 241-298 MOTIF: proline residue (SEVERAL) Ser phosphorylation site {S251} Ser phosphorylation site {S266} Ser phosphorylation site {S287} Ser phosphorylation site {S289}

Database Correlations

OMIM 606447 UniProt Q15287 Pfam PF00076 Entrez Gene 10921 Kegg hsa:10921

References

UniProt :accession Q15287