ribosome biogenesis inhibitor MINAS-60 (MINAS-60)

Function: - acts as a late-stage inhibitor of pre-60S ribosome assembly by preventing pre-60S ribosome export from nucleus - interacts with 60S ribosome assembly factors GTPBP4 & MRTO4 Compartment: - nucleus, nucleolus Alternative splicing: alternative initiation; named isoforms=6 Expression: - increases at early S phase & peaks during S phase - decreases by late S phase & is very low during G2/M - at G1, it again increases Notes: - MINAS-60 is the product of an alternative open reading frame (alt-ORF) of transcripts coding for the RBM10 (AC P98175) protein - MINAS-60 & RBM10 ORFs are overlapping & are formed by shifting the reading frame

General

C2H2 type zinc finger protein nuclear protein phosphoprotein RNA-binding motif protein

Properties

SIZE: entity length = 130 aa MW = 14 kD COMPARTMENT: cell nucleus FORM: ribosome biogenesis inhibitor MINAS-60 MOTIF: Ser phosphorylation site {S50} Ser phosphorylation site {S60} Ser phosphorylation site {S61} Ser phosphorylation site {S65} arginine-rich region {80-87} MOTIF: arginine residue (SEVERAL) Ser phosphorylation site {S89} Thr phosphorylation site {T91} glutamate-rich region {113-125} MOTIF: glutamate residue (SEVERAL) RNP motif NAME: RNP motif SITE: 129-209 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Zinc finger NAME: Zinc finger SITE: 212-242 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S288} RNP motif NAME: RNP motif SITE: 300-384 FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif NAME: ribonucleoprotein-1 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif ribonucleoprotein-2 motif FOR-BINDING-OF: ribonucleic acid MOTIF: ribonucleoprotein-1 motif ribonucleoprotein-2 motif Ser phosphorylation site {S723} Ser phosphorylation site {S733} Ser phosphorylation site {S736} Ser phosphorylation site {S738} Zn finger C2H2-type SITE: 759-784 EFFECTOR-BOUND: Zn+2 Ser phosphorylation site {S797} G-patch domain {858-904}

References

UniProt :accession P0DW28