ribonucleic acid (RNA)

Cellular RNA consists of: 1) rRNA (80%) 2) mRNA (polyA+ RNA is 2-6% of total RNA) 3) tRNA Yield of total RNA varies widely from from tissue & cells. Quantitation of RNA by ultraviolet absorbance: 1) E(260) is 40 mL/ug cm, thus 40 x absorbance at 260 nm gives concentration of RNA (assuming purity) 2) the ratio of A(260)/A(280) is a measure of RNA purity; - values of 1.7-2.1 are generally acceptable Qualitative assessment of RNA by agarose gel electrophoresis: - inspection for 28S & 18S rRNA - band intensity of 28S/18S rRNA should be > 2

Specific

antigene RNA (agRNA) complementary RNA (cRNA) double-stranded RNA (dsRNA) heterologous nuclear RNA (hnRNA) messenger RNA non-coding RNA (ncRNA) Piwi-interacting RNA ribosomal RNA (rRNA) RMRP RNA small nuclear RNA (snRNA) synthetic/pharmaceutical RNA telomerase RNA template component (TER, TERC) transfer RNA (tRNA) vault RNA (vRNA) Y-RNA

Properties

References

Ambion RNAqueous Kit Instruction Manual