Rho guanine nucleotide exchange factor 18 (114 kD Rho-specific guanine nucleotide exchange factor, p114-Rho-GEF, p114RhoGEF, septin-associated RhoGEF, SA-RhoGEF, ARHGEF18, KIAA0521)

Function: - acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases - may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers - also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS) - does not act as a GEF for CDC42 - the G protein beta-gamma (G beta-gamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA & other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change & ROS production - interacts with SEPT9; interaction may inhibit GEF activity - interacts with G beta-gamma subunits GNB1 & GNG2 Structure: - contains 1 DH (DBL-homology) domain - contains 1 PH domain Compartment: - cytoplasm - colocalizes with actin stress fibers Alternative splicing: named isoforms=3 Expression: - expressed in all tissues tested - expresses in kidney & pancreas > liver, skeletal muscle & testis

General

guanine nucleotide exchange factor (GDP/GTP dissociation stimulator, guanine nucleotide-releasing factor/protein, GNEF, GNDS, GDS) phosphoprotein septin

Properties

SIZE: entity length = 1173 aa MW = 131 kD COMPARTMENT: cytoplasm MOTIF: Dbl homology domain {259-456} PH domain {496-598} coiled coil {799-819} coiled coil {850-960} Ser phosphorylation site {S1103} proline-rich region SITE: 1104-1158 MOTIF: proline residue (SEVERAL)

Database Correlations

UniProt Q6ZSZ5 PFAM correlations

References

UniProt :accession Q6ZSZ5