retina-specific copper amine oxidase; RAO; amine oxidase [copper-containing]; semicarbazide-sensitive amine oxidase; SSAO (AOC2)

Function: 1) modulates signal transmission in retina 2) degrades biogenic amines dopamine, histamine, putrescine 3) may play a role in adipogenesis 4) topaquinone is generated by copper-dependent autoxidation of a specific Tyr 5) forms heterodimer with AOC3, in vitro RCH2NH2 + H₂O + O₂ RCHO + NH2 + H₂O₂ Cofactor: binds 1 Cu+1/Cu+2 ion per subunit binds 2 Ca⁺² per subunit contains 1 topaquinone per subunit Kinetic parameters: - KM=0.056 mM for tryptamine - KM=0.077 mM for 2-phenylethylamine - KM=0.167 mM for benzylamine - KM=0.178 mM for p-tyramine - KM=1.7 mM for methylamine Structure: belongs to the copper/topaquinone oxidase family Compartment: - isoform 1: cell membrane - isoform 2: cytoplasm Alternative splicing: - named isoforms=2 - additional isoforms seem to exist Expression: - expressed in many tissues - much higher expression in retina - isoform 1 & isoform 2 are expressed in adipose tissue - up-regulated during in vitro adipocyte differentiation - isoform 1 only seems to be expressed in thymus - isoform 2 only expressed in testis

General

amine oxidoreductase Ca+2 binding protein glycoprotein

Properties

SIZE: MW = 84 kD entity length = 756 aa MOTIF: signal sequence {1-32} N-glycosylation site {N133} N-glycosylation site {N198} N-glycosylation site {N226} aspartate residue {D380} tyrosine residue {Y465} copper [Cu]-binding site SITE: 516-516 MOTIF: histidine residue (3) copper [Cu]-binding site SITE: 518-518 MOTIF: histidine residue (3) Ca+2-binding site SITE: 525-525 Ca+2-binding site SITE: 526-526 Ca+2-binding site SITE: 527-527 Ca+2-binding site SITE: 568-568 N-glycosylation site {N588} Ca+2-binding site SITE: 634-634 Ca+2-binding site SITE: 659-659 Ca+2-binding site SITE: 661-661 N-glycosylation site {N662} Ca+2-binding site SITE: 663-663 Ca+2-binding site SITE: 669-669 Ca+2-binding site SITE: 670-670 copper [Cu]-binding site SITE: 680-680 MOTIF: histidine residue (3)

Database Correlations

OMIM 602268 UniProt O75106 PFAM correlations Entrez Gene 314 Kegg hsa:314 ENZYME 1.4.3.21