replication initiation-like protein (myelin transcription factor 2, MyT2, cerebrin-50, MYT2)

Function: - may be involved in DNA replication - binds to the promoter of the myelin proteolipid protein PLP1 gene - preferentially binds supercoiled DNA - reduces the number of supercoils in a highly negatively supercoiled DNA via a non-enzymatic mechanism - binds also to methylated & non-methylated single-stranded oligonucleotides corresponding to the Sp1 binding site of HIV-1 LTR Structure: - highly similar to microbial proteins Compartment: - nucleus, cytoplasm, secreted - detected in cerebrospinal fluid & in T-lymphoblastoid cell culture medium & serum Alternative splicing: named isoforms=2 Expression: - expressed in brain - expressed in oligodendrocyte progenitor cells, pyramidal cells & purkinje neurons, neurons of the hypothalamus, hippocampus, ventromedial thalamus, cells of the choroid plexus & ciliated ependymal cells (at protein level) - expressed predominantly in brain & a T-lymphoblastoid cell line, moderately in spleen, liver & testis - mRNA transcript encoding MYT2 contains an internal ribosome entry site (IRES) in order to promote its translation - belongs to the low-abundance category of cellular transcripts undetectable by Northern blot or Rnase protection assays Note: - MYT2 has not yet been found to be encoded in the human genome

Properties

SIZE: entity length = 435 aa MW = 51 kD COMPARTMENT: cytoplasm cell nucleus MOTIF: coiled coil {348-378}

Database Correlations

OMIM 603677 Pfam PF02486